Nature Communications (Dec 2022)
A genome-wide CRISPR screen identifies WDFY3 as a regulator of macrophage efferocytosis
- Jianting Shi,
- Xun Wu,
- Ziyi Wang,
- Fang Li,
- Yujiao Meng,
- Rebecca M. Moore,
- Jian Cui,
- Chenyi Xue,
- Katherine R. Croce,
- Arif Yurdagul,
- John G. Doench,
- Wei Li,
- Konstantinos S. Zarbalis,
- Ira Tabas,
- Ai Yamamoto,
- Hanrui Zhang
Affiliations
- Jianting Shi
- Cardiometabolic Genomics Program, Division of Cardiology, Department of Medicine, Columbia University Irving Medical Center
- Xun Wu
- Cardiometabolic Genomics Program, Division of Cardiology, Department of Medicine, Columbia University Irving Medical Center
- Ziyi Wang
- Cardiometabolic Genomics Program, Division of Cardiology, Department of Medicine, Columbia University Irving Medical Center
- Fang Li
- Cardiometabolic Genomics Program, Division of Cardiology, Department of Medicine, Columbia University Irving Medical Center
- Yujiao Meng
- Cardiometabolic Genomics Program, Division of Cardiology, Department of Medicine, Columbia University Irving Medical Center
- Rebecca M. Moore
- Cardiometabolic Genomics Program, Division of Cardiology, Department of Medicine, Columbia University Irving Medical Center
- Jian Cui
- Cardiometabolic Genomics Program, Division of Cardiology, Department of Medicine, Columbia University Irving Medical Center
- Chenyi Xue
- Cardiometabolic Genomics Program, Division of Cardiology, Department of Medicine, Columbia University Irving Medical Center
- Katherine R. Croce
- Department of Pathology and Cell Biology, Columbia University
- Arif Yurdagul
- Department of Molecular & Cellular Physiology, Louisiana State University Health Sciences Center at Shreveport
- John G. Doench
- Broad Institute of MIT and Harvard
- Wei Li
- Center for Genetic Medicine Research, Children’s National Hospital
- Konstantinos S. Zarbalis
- University of California at Davis, Department of Pathology and Laboratory Medicine
- Ira Tabas
- Department of Pathology and Cell Biology, Columbia University
- Ai Yamamoto
- Department of Pathology and Cell Biology, Columbia University
- Hanrui Zhang
- Cardiometabolic Genomics Program, Division of Cardiology, Department of Medicine, Columbia University Irving Medical Center
- DOI
- https://doi.org/10.1038/s41467-022-35604-8
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 19
Abstract
Efferocytosis describes the engulfment and clearance of apoptotic cells by phagocytes. Here the authors identify in primary mouse macrophage WDFY3 as a regulator for efferocytosis, in which c-terminal WDFY3 is sufficient to modulate degradation while full-length WDFY3 is required to modulate the uptake of apoptotic cells.
