Cas9 targeted enrichment of mobile elements using nanopore sequencing

Torrin L. McDonald; Weichen Zhou; Christopher P. Castro; Camille Mumm; Jessica A. Switzenberg; Ryan E. Mills; Alan P. Boyle

doi:10.1038/s41467-021-23918-y

Nature Communications (Jun 2021)

Cas9 targeted enrichment of mobile elements using nanopore sequencing

Torrin L. McDonald,
Weichen Zhou,
Christopher P. Castro,
Camille Mumm,
Jessica A. Switzenberg,
Ryan E. Mills,
Alan P. Boyle

Affiliations

Torrin L. McDonald: Department of Human Genetics, University of Michigan
Weichen Zhou: Department of Computational Medicine and Bioinformatics, University of Michigan
Christopher P. Castro: Department of Computational Medicine and Bioinformatics, University of Michigan
Camille Mumm: Department of Human Genetics, University of Michigan
Jessica A. Switzenberg: Department of Computational Medicine and Bioinformatics, University of Michigan
Ryan E. Mills: Department of Human Genetics, University of Michigan
Alan P. Boyle: Department of Human Genetics, University of Michigan

DOI: https://doi.org/10.1038/s41467-021-23918-y
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 13

Abstract

Read online

Mobile element insertions (MEIs) are a source of repetitive genetic variation and can lead to genetic disorders. Here the authors use Cas9-targeted nanopore sequencing to efficiently saturate enrichment for known and non-reference MEIs.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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