Nature Communications (Dec 2022)

A quantitative model for the dynamics of target recognition and off-target rejection by the CRISPR-Cas Cascade complex

  • Marius Rutkauskas,
  • Inga Songailiene,
  • Patrick Irmisch,
  • Felix E. Kemmerich,
  • Tomas Sinkunas,
  • Virginijus Siksnys,
  • Ralf Seidel

DOI
https://doi.org/10.1038/s41467-022-35116-5
Journal volume & issue
Vol. 13, no. 1
pp. 1 – 13

Abstract

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Based on thorough single-molecule analysis of off-target binding by the CRISPR-Cas surveillance complex Cascade, a mechanism-based model is presented that quantitatively describes the recognition dynamics of mutated targets by this protein.