CTGF and MLH1 Gene Expression Levels in Colorectal Cancer Tumor Tissues and Adjacent Normal Tissues in Patients in Golestan Province

Abstract

Background and purpose: Colorectal cancer is the third most common type of cancer in terms of incidence and the second most common cause of cancer-related death worldwide. The aim of this study was to investigate the expression of CTGF and MLH1 gene in colorectal cancer tumor tissues and adjacent normal tissues in patients in Golestan province. Materials and methods: In this experimental study, 20 colorectal cancer tissue samples and 20 adjacent healthy tissue samples were collected from Sayad Shirazi Hospital Research Center, Gorgan. After RNA extraction, cDNA synthesis and primer design, the expresion of CTGF and MLH1 genes were measured by Real Time PCR. Statistical analysis of data was done in Graphpad Prism software. Results: According to findings, increase was seen in the CTGF gene expression level in tumor tissue to the adjacent healthy tissue (P<0.05), while the ratio of the MLH1 gene expression in the tumor tissue to the adjacent healthy tissue decreased (P<0.05). Conclusion: This study showed that increased expression levels of CTGF gene and decreased expression levels of MLH1 gene play roles in colorectal cancer.

Keywords

• colorectal cancer
• gene expression
• ctgf
• mlh1
• golestan province