Acute, Subacute and Subchronic Toxicological Studies of Carissa Carandas Leaves (Ethanol Extract): A Plant Active against Cardiovascular Diseases

Abstract

Objective: The Purpose of this research study was to examine the toxicological effects of aqueous: ethanol(1:1) extract of Carissa carandas leaves extracts in rats. Methodolgy: Acute toxicity studies were conducted to check the LD50 values in experimental animals. Autopsy after acute toxicity revealed that no gross changes were observed in organs like liver, spleen, heart and kidney among the animals of group N (control) and S (treated). The appearance of organs of Group S animals was comparable with that of Group N animals. Results: No signs of toxicity and mortality were observed in treated group after sub acute toxicity as comparedto the control group. The histopathological studies after subchronic toxicity in doses of 1750mg/kg (p.o.) and5000mg/kg (p.o) showed no toxic effects on organs like liver, heart, kidney and spleen. While chronic toxicity in dose 5000mg/kg (p.o.) showed some histological changes.

Keywords

• carissa carandas
• acute toxicity
• subacute toxicity
• chronic toxicity