Ophthalmology and Therapy (Oct 2024)

Intraretinal Microvascular Abnormalities in Eyes with Advanced Stages of Nonproliferative Diabetic Retinopathy: Comparison Between UWF-FFA, CFP, and OCTA—The RICHARD Study

  • Ana R. Santos,
  • Marta Lopes,
  • Torcato Santos,
  • Débora Reste-Ferreira,
  • Inês P. Marques,
  • Taffeta C. N. Yamaguchi,
  • Telmo Miranda,
  • Luís Mendes,
  • António C. V. Martinho,
  • Liz Pearce,
  • José Cunha-Vaz

DOI
https://doi.org/10.1007/s40123-024-01054-2
Journal volume & issue
Vol. 13, no. 12
pp. 3161 – 3173

Abstract

Read online

Abstract Introduction This study aimed to evaluate intraretinal microvascular abnormalities (IRMA) in eyes with advanced nonproliferative diabetic retinopathy (NPDR) using multimodal approach in co-located areas focusing on central retina (up to 50°) and to look at possible correlations between IRMA and other structural changes, like ischemia and presence of microaneurysms. Methods The RICHARD study (NCT05112445) included 60 eyes from 60 patients with type 2 diabetes with moderate-severe NPDR, diabetic retinopathy severity levels 43, 47, and 53 (DRSS). IRMA were defined as capillary tortuosity covering a minimum circular area of 300 µm (calculated to correspond to the Early Treatment Diabetic Retinopathy Study standard photo 8A) and were identified using multimodal imaging with distinct fields of view (FoV): color fundus photography (CFP) using a Topcon TRC-50DX camera (Topcon Medical Systems, Japan), Optos California ultra wide field fundus fluorescein angiography (UWF-FFA) (Optos plc, UK), and swept-source optical coherence tomography angiography (SS-OCTA) (PLEX® Elite 9000, ZEISS, USA). Different areas of the retina were examined: central macula (up to 20°) and posterior pole (between 20° and 50°). Results Multimodal imaging was used to identify IRMA in co-located areas (FoV 20°). Our results show that when evaluating the presence of IRMA (FoV < 50°), UWF-FFA detected 203 IRMA, SS-OCTA detected 133 IRMA, and CFP detected 104 IRMA. Our results also show that the presence of IRMA was positively associated with presence of microaneurysms. Conclusions Identification of IRMA in eyes with advanced NPDR is better achieved by UWF-FFA than CFP and SS-OCTA. A statistically significant correlation was found between the presence of IRMA and the increase in number of microaneurysms. Trial Registration ClinicalTrials.gov, identifier NCT05112445.

Keywords