Frontiers in Genetics (Jan 2023)

Mapping of a QTL associated with sucrose content in peanut kernels using BSA-seq

  • Junjia Guo,
  • Feiyan Qi,
  • Li Qin,
  • Maoning Zhang,
  • Ziqi Sun,
  • Hongyan Li,
  • Mengjie Cui,
  • Mengyuan Zhang,
  • Chenyu Li,
  • Xiaona Li,
  • Qi Zhao,
  • Dandan Luo,
  • Mengdi Tian,
  • Hua Liu,
  • Jing Xu,
  • Lijuan Miao,
  • Bingyan Huang,
  • Wenzhao Dong,
  • Suoyi Han,
  • Xinyou Zhang

DOI
https://doi.org/10.3389/fgene.2022.1089389
Journal volume & issue
Vol. 13

Abstract

Read online

As an important factor affecting the edible quality of peanut kernels, sucrose content is a complex quantitative trait regulated by multiple factors. In this study, an F2 segregating population and a recombinant inbred line (RIL) population, derived from a cross between the high sucrose content variety Jihuatian 1 and the low sucrose content line PI478819, were used as materials to map a quantitative trait locus (QTL) associated with sucrose content in peanut kernels. Four QTLs were initially located on chromosomes A03 and A06 based on BSA-seq technology, and multiple kompetitive allele-specific PCR markers were developed based on single-nucleotide polymorphisms (SNPs) in the intervals. The markers were genotyped in the RIL population and finely mapped to a stable QTL, qSUCA06, located on chromosome A06 within a 0.29-Mb physical genomic interval (112367085–112662675 bp), which accounted for 31.95%–41.05% of the phenotypic variance explained. SNP and insertion/deletion annotations were performed on genes in the candidate interval, and having screened out those genes with mutations in exons, candidate genes were verified by qRT-PCR. The results revealed that Arahy.Y2LWD9 may be the main gene regulating sucrose content. The QTL identified in this study will not only contribute to marker-assisted breeding for improvement of peanut sucrose content but also paves the way for identifying gene function.

Keywords