Generation of the induced pluripotent stem cell line UHOMi002-A from peripheral blood mononuclear cells of a healthy male donor

Mathieu Fieldes; Engi Ahmed; Chloé Bourguignon; Joffrey Mianné; Mélanie Martin; Cécile Arnould; Isabelle Vachier; Said Assou; John De Vos; Arnaud Bourdin

Stem Cell Research (Dec 2020)

Generation of the induced pluripotent stem cell line UHOMi002-A from peripheral blood mononuclear cells of a healthy male donor

Mathieu Fieldes,
Engi Ahmed,
Chloé Bourguignon,
Joffrey Mianné,
Mélanie Martin,
Cécile Arnould,
Isabelle Vachier,
Said Assou,
John De Vos,
Arnaud Bourdin

Affiliations

Mathieu Fieldes: IRMB, Univ Montpellier, INSERM, CHU Montpellier, Montpellier 34000, France
Engi Ahmed: IRMB, Univ Montpellier, INSERM, CHU Montpellier, Montpellier 34000, France; Department of Respiratory Diseases, CHU Montpellier, Arnaud de Villeneuve Hospital, INSERM, Montpellier 34000, France
Chloé Bourguignon: IRMB, Univ Montpellier, INSERM, CHU Montpellier, Montpellier 34000, France
Joffrey Mianné: IRMB, Univ Montpellier, INSERM, CHU Montpellier, Montpellier 34000, France
Mélanie Martin: Laboratory of Molecular Genetics, Univ Montpellier, CHU Nîmes, Nîmes, France
Cécile Arnould: Laboratory of Molecular Genetics, Univ Montpellier, CHU Nîmes, Nîmes, France
Isabelle Vachier: Department of Respiratory Diseases, CHU Montpellier, Arnaud de Villeneuve Hospital, INSERM, Montpellier 34000, France
Said Assou: IRMB, Univ Montpellier, INSERM, CHU Montpellier, Montpellier 34000, France
John De Vos: IRMB, Univ Montpellier, INSERM, CHU Montpellier, Montpellier 34000, France; Department of Cell and Tissue Engineering, Saint-Eloi Hospital, Montpellier 34000, France; Corresponding authors at: Department of Cell and Tissue Engineering, Saint-Eloi Hospital, 80 Avenue Augustin Fliche, 34000 Montpellier, France (D.V. John). Department of Respiratory Diseases, Montpellier University Hospitals, Arnaud de Villeneuve Hospital, 371 Av. du Doyen Gaston Giraud, 34090, Montpellier, France (B. Arnaud).
Arnaud Bourdin: Department of Respiratory Diseases, CHU Montpellier, Arnaud de Villeneuve Hospital, INSERM, Montpellier 34000, France; PhyMedExp, University of Montpellier, INSERM U1046, CNRS UMR 9214; Corresponding authors at: Department of Cell and Tissue Engineering, Saint-Eloi Hospital, 80 Avenue Augustin Fliche, 34000 Montpellier, France (D.V. John). Department of Respiratory Diseases, Montpellier University Hospitals, Arnaud de Villeneuve Hospital, 371 Av. du Doyen Gaston Giraud, 34090, Montpellier, France (B. Arnaud).

Journal volume & issue: Vol. 49
p. 102037

Abstract

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Human pluripotent stem cells (hiPSC) are highly valuable tools to model lung development and chronic bronchial diseases. We generated a hiPSC line from a highly characterized 40-year-old healthy male nonsmoking donor. Peripheral blood mononuclear cells (PBMCs) were reprogrammed using integration-free Sendai Virus. The cell line had normal karyotype, expressed pluripotency hallmarks, and differentiated into the three primary germ layers.The reported UHOMi002-A iPSC line may be used as a control to model lung development, study human chronic bronchial diseases and drug testing.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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