Efficient organogenesis and taxifolin production system from mature zygotic embryos and needles in larch

Abstract

The deciduous conifer larch has been widely distributed around the world, is a high-quality wood species and is also used to extract industrial raw materials and medicines. In this study, we developed an organogenesis protocol for Larix olgensis from both mature zygotic embryos and needles, and analyzed the content of taxifolin in different tissues. The highest callus induction (96.8%) from mature zygotic embryo was found in the Douglas-fir Cotyledon Revised (DCR) medium augmented with 2.0 mg·L−1 6-Benzylaminopurine (6-BA) and 0.2 mg·L−1 α-Naphthaleneacetic acid (NAA), while from needles the highest callus induction (92.03%) was found in the Murashige and Skoog (MS) medium augmented with 3 mg·L−1 6-BA and 0.3 mg·L−1 NAA. The best shoot regeneration capacity from zygotic embryo-derived calli (83.3%) was obtained in DCR medium augmented with 1.0 mg·L−1 6-BA and 0.01 mg·L−1 NAA, and needle-derived calli were 77.3%. The shoots achieved the highest elongation (75.6%) in the DCR medium supplemented with 0.5 mg·L−1 6-BA, 0.05 mg·L−1 NAA and 2 g·L−1 activated charcoal (AC). The rooting rate was 62.8% in DCR medium augmented with 3 mg·L−1 Indole-3-butyric acid (IBA) and 100 mg·L−1 phloroglucinol (PG). The accumulation of the taxifolin in elongation shoots and lignified elongation shoots have greatly improved along with the development process, were 28.6 µg·g−1, and 53 µg·g−1 respectively. The content of the taxifolin in callus was 1.99−5.26 µg·g−1, adventitious shoots were 4.8 µg·g−1, and adventitious roots were 2.86 µg·g−1. We report an efficient organogenesis and taxifolin production protocol in larch for the first time.

Keywords

• larix olgensis
• mature zygotic embryo
• needle
• organogenesis
• taxifolin production