Tolerance of blackgrass (Alopecurus myosuroide) to sylfonylurea herbicides in the Czech Republic

Abstract

Seeds of three blackgrass populations fromSouthern Bohemiawere collected in 2007-2008. Biological tests with chlorsulfuron were performed at the 2-3 leaf stage. Some plants survived after treatment with the highest dose 37.5 g/ha. Biological tests showed a resistant phenotype to chlorsulfuron. Leaves of these plants were analysed by dCAPS assay. Two domains of ALS gene: domain A ‒ P197 and domain B ‒ W574 were targeted by PCR with regenerated primers P197 containing BamHI site and W574 containing site BstXI. PCR products of all tested samples were cleaved by BamHI in the codon P197. No mutation of proline in P197 was found out. The codon W574 PCR product of the samples was not cleaved by BstXI.

Keywords

• alopecurus myosuroides
• als
• resistance
• sulfonylurea
• dcaps