Antibody screening data of human midgestation liver cells with a focus on hematopoietic, liver sinusoidal endothelial, and hepatoblast cell-populations

Abstract

Abstract Objectives Cell-surface antigen screening was performed on human fetal liver cells using flow cytometry. The goal was to provide proteomic expression data on a number of human fetal liver cell populations that can inform studies on developmental hepatology and hematology. Data description A 21 weeks’ gestation liver was depleted of erythrocytes prior to antibody staining. Screening was performed using phycoerythrin-labelled antibodies against 332 antigens. In addition to these antibodies, all samples were stained for CD14, CD45, CD235a, and CD326 (epithelial cell adhesion molecule – EpCAM). Subpopulations of fetal liver cells were identified using the co-stained antigens. Hematopoietic cells were identified by their expression of CD45 and CD235a; non-hematopoietic cells were further subdivided based on CD14 and CD326 expression. CD326++CD14low hepatoblasts and CD14++ liver sinusoidal endothelial cells were analyzed for the frequency and intensity of antigen expression. Analyzed flow cytometry data are presented for the expression of the antigens on hematopoietic cells and on non-hematopoietic cells in the context of CD14 and CD326 expression.

Keywords

• Flow cytometry
• Antigens
• Hepatology
• Liver
• Endothelial Cells
• Blood Cells