Optimized protocol for naive human pluripotent stem cell-derived trophoblast induction

Shingo Io; Yoshiki Iemura; Yasuhiro Takashima

STAR Protocols (Dec 2021)

Optimized protocol for naive human pluripotent stem cell-derived trophoblast induction

Shingo Io,
Yoshiki Iemura,
Yasuhiro Takashima

Affiliations

Shingo Io: Department of Life Science Frontiers, CiRA, Kyoto University, Kyoto 606-8507, Japan; Department of Gynecology and Obstetrics, Kyoto University Graduate School of Medicine, Kyoto 606-8507, Japan; Department of Obstetrics and Gynecology, Kosaka Women’s Hospital, Osaka 577-0807, Japan; Corresponding author
Yoshiki Iemura: Department of Life Science Frontiers, CiRA, Kyoto University, Kyoto 606-8507, Japan; Optional Department of Diagnostic Pathology, Kyoto University Graduate School of Medicine, Kyoto 606-8507, Japan; Japan Society for the Promotion of Science, Tokyo 102-0083, Japan
Yasuhiro Takashima: Department of Life Science Frontiers, CiRA, Kyoto University, Kyoto 606-8507, Japan; Corresponding author

Journal volume & issue: Vol. 2, no. 4
p. 100921

Abstract

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Summary: Human trophoblasts arise from the morula as trophectoderm, which differentiates into cytotrophoblast, syncytiotrophoblast, and extravillous trophoblast after implantation. Here, we present a robust step-by-step protocol to induce trophectoderm (TE) from naive human pluripotent stem cells (PSCs) corresponding to pre-implantation epiblast. Our culture system (TE induction and ACE condition) mimics the entire trophoblast development including the molecular events.For complete details on the use and execution of this protocol, please refer to Io et al. (2021).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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