Efficiency of Vanilla, Patchouli and Ylang Ylang Essential Oils Stabilized by Iron Oxide@C14 Nanostructures against Bacterial Adherence and Biofilms Formed by Staphylococcus aureus and Klebsiella pneumoniae Clinical Strains

Maxim Bilcu; Alexandru Mihai Grumezescu; Alexandra Elena Oprea; Roxana Cristina Popescu; George Dan Mogoșanu; Radu Hristu; George A. Stanciu; Dan Florin Mihailescu; Veronica Lazar; Eugenia Bezirtzoglou; Mariana Carmen Chifiriuc

doi:10.3390/molecules191117943

Molecules (Nov 2014)

Efficiency of Vanilla, Patchouli and Ylang Ylang Essential Oils Stabilized by Iron Oxide@C14 Nanostructures against Bacterial Adherence and Biofilms Formed by Staphylococcus aureus and Klebsiella pneumoniae Clinical Strains

Maxim Bilcu,
Alexandru Mihai Grumezescu,
Alexandra Elena Oprea,
Roxana Cristina Popescu,
George Dan Mogoșanu,
Radu Hristu,
George A. Stanciu,
Dan Florin Mihailescu,
Veronica Lazar,
Eugenia Bezirtzoglou,
Mariana Carmen Chifiriuc

Affiliations

Maxim Bilcu: Microbiology Department, Faculty of Biology, Research Institute of the University of Bucharest, University of Bucharest, Aleea Portocalelor No. 1–3, 060101 Bucharest, Romania
Alexandru Mihai Grumezescu: Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, Polizu Street No. 1–7, 011061 Bucharest, Romania
Alexandra Elena Oprea: Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, Polizu Street No. 1–7, 011061 Bucharest, Romania
Roxana Cristina Popescu: Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, Polizu Street No. 1–7, 011061 Bucharest, Romania
George Dan Mogoșanu: Department of Pharmacognosy & Phytotherapy, Faculty of Pharmacy, University of Medicine and Pharmacy of Craiova, 2 Petru Rareş Street, 200349 Craiova, Romania
Radu Hristu: Center for Microscopy-Microanalysis and Information Processing, University Politehnica of Bucharest, 313 Splaiul Independentei, 060042 Bucharest, Romania
George A. Stanciu: Center for Microscopy-Microanalysis and Information Processing, University Politehnica of Bucharest, 313 Splaiul Independentei, 060042 Bucharest, Romania
Dan Florin Mihailescu: Microbiology Department, Faculty of Biology, Research Institute of the University of Bucharest, University of Bucharest, Aleea Portocalelor No. 1–3, 060101 Bucharest, Romania
Veronica Lazar: Microbiology Department, Faculty of Biology, Research Institute of the University of Bucharest, University of Bucharest, Aleea Portocalelor No. 1–3, 060101 Bucharest, Romania
Eugenia Bezirtzoglou: Laboratory of Microbiology, Biotechnology and Hygiene, Department of Food Science and Technology, Faculty of Agricultural Development, Democritus University of Thrace, 68200 Orestiada, Greece
Mariana Carmen Chifiriuc: Microbiology Department, Faculty of Biology, Research Institute of the University of Bucharest, University of Bucharest, Aleea Portocalelor No. 1–3, 060101 Bucharest, Romania

DOI: https://doi.org/10.3390/molecules191117943
Journal volume & issue: Vol. 19, no. 11
pp. 17943 – 17956

Abstract

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Biofilms formed by bacterial cells are associated with drastically enhanced resistance against most antimicrobial agents, contributing to the persistence and chronicization of the microbial infections and to therapy failure. The purpose of this study was to combine the unique properties of magnetic nanoparticles with the antimicrobial activity of three essential oils to obtain novel nanobiosystems that could be used as coatings for catheter pieces with an improved resistance to Staphylococcus aureus and Klebsiella pneumoniae clinical strains adherence and biofilm development. The essential oils of ylang ylang, patchouli and vanilla were stabilized by the interaction with iron oxide@C14 nanoparticles to be further used as coating agents for medical surfaces. Iron oxide@C14 was prepared by co-precipitation of Fe+2 and Fe+3 and myristic acid (C14) in basic medium. Vanilla essential oil loaded nanoparticles pelliculised on the catheter samples surface strongly inhibited both the initial adherence of S. aureus cells (quantified at 24 h) and the development of the mature biofilm quantified at 48 h. Patchouli and ylang-ylang essential oils inhibited mostly the initial adherence phase of S. aureus biofilm development. In the case of K. pneumoniae, all tested nanosystems exhibited similar efficiency, being active mostly against the adherence K. pneumoniae cells to the tested catheter specimens. The new nanobiosystems based on vanilla, patchouli and ylang-ylang essential oils could be of a great interest for the biomedical field, opening new directions for the design of film-coated surfaces with anti-adherence and anti-biofilm properties.

Published in Molecules

ISSN: 1420-3049 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Chemistry: Organic chemistry
Website: http://www.mdpi.com/journal/molecules

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