Phytomediated synthesis of bimetallic Ag/Au nanoparticles using orange peel extract and assessment of their antibacterial and anitcancer potential

Abstract

The current research interest in nanoscience centers around the synthesis of nanoparticle with noble metals due to their distinctive characteristics that has expanded their prospective utilization in various fields of science and technology. The study reports the green fabrication of bimetallic composite nanoparticles (CNPs) with silver (Ag) and gold(Au) via an eco-friendly mode with a biomaterial, orange peel (OP) extract as a reductant. The reaction of aqueous solutions of silver nitrate (AgNO3) and chloroauric acid (HAuCl4) with the OP extract resulted in rapid production of stable CNPs having spectacular spherical shapes and other morphologies. The characterization of the synthesized CNPs included the following analyses; UV–vis spectrophotometer, (DLS) dynamic light scattering technique for the particles average size was found to be 138 nm with a polydispersity index (PDI) of 0.249. To identify the functional groups in biomolecules that emphasizes the reducing and capping agent for NPs, the Fourier transform infrared spectroscopy (FTIR) has been used. The characterization of as prepared NPs has been complemented with (TEM) transmission Electron Microscopy, and (SEM) scanning Electron Microscopy along with the (EDS) energy dispersive spectrometry for the elemental screening of the sample. Furthermore, antibacterial effect and the cytotoxicity activity against the human MDA cell line of the bimetallic CNPs were investigated. The key findings showed that the CNPs suspension possesses potent cytotoxic activity (and was highly effective against bacterial strains. Thus, the finding support the prospective therapeutic potential of the nanoparticles in nano medicine.

Keywords

• Bimetallic nanoparticles
• Green Synthesis
• Orange peel extract
• Cytotoxic activity and Antibacterial activity