Functional cardiac fibroblasts derived from human pluripotent stem cells via second heart field progenitors

Jianhua Zhang; Ran Tao; Katherine F. Campbell; Juliana L. Carvalho; Edward C. Ruiz; Gina C. Kim; Eric G. Schmuck; Amish N. Raval; André Monteiro da Rocha; Todd J. Herron; José Jalife; James A. Thomson; Timothy J. Kamp

doi:10.1038/s41467-019-09831-5

Nature Communications (May 2019)

Functional cardiac fibroblasts derived from human pluripotent stem cells via second heart field progenitors

Jianhua Zhang,
Ran Tao,
Katherine F. Campbell,
Juliana L. Carvalho,
Edward C. Ruiz,
Gina C. Kim,
Eric G. Schmuck,
Amish N. Raval,
André Monteiro da Rocha,
Todd J. Herron,
José Jalife,
James A. Thomson,
Timothy J. Kamp

Affiliations

Jianhua Zhang: Department of Medicine, School of Medicine and Public Health, University of Wisconsin-Madison
Ran Tao: Department of Medicine, School of Medicine and Public Health, University of Wisconsin-Madison
Katherine F. Campbell: Center for Arrhythmia Research, Department of Internal Medicine, Cardiovascular Medicine, University of Michigan
Juliana L. Carvalho: Department of Medicine, School of Medicine and Public Health, University of Wisconsin-Madison
Edward C. Ruiz: Department of Medicine, School of Medicine and Public Health, University of Wisconsin-Madison
Gina C. Kim: Department of Medicine, School of Medicine and Public Health, University of Wisconsin-Madison
Eric G. Schmuck: Department of Medicine, School of Medicine and Public Health, University of Wisconsin-Madison
Amish N. Raval: Department of Medicine, School of Medicine and Public Health, University of Wisconsin-Madison
André Monteiro da Rocha: Center for Arrhythmia Research, Department of Internal Medicine, Cardiovascular Medicine, University of Michigan
Todd J. Herron: Center for Arrhythmia Research, Department of Internal Medicine, Cardiovascular Medicine, University of Michigan
José Jalife: Center for Arrhythmia Research, Department of Internal Medicine, Cardiovascular Medicine, University of Michigan
James A. Thomson: Regenerative Biology Division, Morgridge Institute for Research
Timothy J. Kamp: Department of Medicine, School of Medicine and Public Health, University of Wisconsin-Madison

DOI: https://doi.org/10.1038/s41467-019-09831-5
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 15

Abstract

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Cardiac fibroblasts (CFs) play critical roles in heart development, homeostasis, and disease. Here the authors efficiently differentiate human pluripotent stem cells through second heart field progenitors to CFs that exhibit features and functional properties similar to native CFs.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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