Xanthine oxidase inhibitory activity of compounds from Chythrantus claneianus

Abstract

Phytochemical investigation of the stem bark and the trunk of Chythrantus claneianus led to the isolation of six known compounds named β-sitosterol (1), umbelliferone (2), scopoletin (3), benjaminamide (4), β-sitosterol-3-O-β-D-glucopyranoside (5) and panconoside B (6). All these compounds were isolated for the first time from this plant species. The chemical structures of isolates were elucidated on the basis of 1 and 2 D-NMR spectra and other spectroscopic techniques including UV–vis, FT-IR, HR-ESIMS and HR-FABMS. The isolates were tested in vitro for their inhibitory properties towards xanthine oxidase enzyme. Compounds 2, 3 and 6 showed weak inhibitory activities on the enzyme with IC50 values ranging from 307 µM for compound 6 to 475 µM for compound 3, while the extract and compounds 1, 4 and 5 showed extremely weak activities with inhibition percentage less than 50%.

Keywords

• Chythrantus claneianus
• rheumatism
• xanthine oxidase inhibitor