STUDY OF THE ROLE OF INNATE IMMUNITY FACTORS (TLR2, HBD-2, TNF-a, TGF-P) IN PERIODONTITIS PATHOGENESIS

Abstract

Aim. Study of the role of innate immunity factors, namely, expression of TLR2 and HBD-2 genes, as well as TNF-a and TGF-P in pathogenesis of periodontium tissue inflammation. Materials and methods. 59 individuals with periodontitis illnesses were included into the study; 15 healthy donors represented the comparison group. Study of the TLR2 and HBD-2 gene expression levels was carried out by rRT-PCR, cytokine production evaluation - by EIA. Results. The results of the study have shown the presence of TLR-mediated disbalance in the innate immunity system in the periodontium tissue during chronic generalized periodontitis. TLR2 gene hyperexpression was accompanied by the reduction of expression of anti-microbial peptide HBD-2, as well as an increase of production of TNF-a and TGF-P by epithelial cells of periodontium mucosa. Conclusion. The study carried out has shown that disturbance of molecular mechanisms of innate immunity system has an important place in pathogenesis of periodontitis.

Keywords

• periodontitis
• toll-like receptors
• defensins
• cytokines
• innate immunity