An engineered CRISPR-Cas12a variant and DNA-RNA hybrid guides enable robust and rapid COVID-19 testing

Kean Hean Ooi; Mengying Mandy Liu; Jie Wen Douglas Tay; Seok Yee Teo; Pornchai Kaewsapsak; Shengyang Jin; Chun Kiat Lee; Jingwen Hou; Sebastian Maurer-Stroh; Weisi Lin; Benedict Yan; Gabriel Yan; Yong-Gui Gao; Meng How Tan

doi:10.1038/s41467-021-21996-6

Nature Communications (Mar 2021)

An engineered CRISPR-Cas12a variant and DNA-RNA hybrid guides enable robust and rapid COVID-19 testing

Kean Hean Ooi,
Mengying Mandy Liu,
Jie Wen Douglas Tay,
Seok Yee Teo,
Pornchai Kaewsapsak,
Shengyang Jin,
Chun Kiat Lee,
Jingwen Hou,
Sebastian Maurer-Stroh,
Weisi Lin,
Benedict Yan,
Gabriel Yan,
Yong-Gui Gao,
Meng How Tan

Affiliations

Kean Hean Ooi: School of Chemical and Biomedical Engineering, Nanyang Technological University
Mengying Mandy Liu: School of Chemical and Biomedical Engineering, Nanyang Technological University
Jie Wen Douglas Tay: School of Chemical and Biomedical Engineering, Nanyang Technological University
Seok Yee Teo: School of Chemical and Biomedical Engineering, Nanyang Technological University
Pornchai Kaewsapsak: Genome Institute of Singapore, Agency for Science Technology and Research
Shengyang Jin: School of Biological Sciences, Nanyang Technological University
Chun Kiat Lee: Department of Laboratory Medicine, National University Hospital, National University Health System
Jingwen Hou: School of Computer Science and Engineering, Nanyang Technological University
Sebastian Maurer-Stroh: Bioinformatics Institute, Agency for Science Technology and Research
Weisi Lin: School of Computer Science and Engineering, Nanyang Technological University
Benedict Yan: Department of Laboratory Medicine, National University Hospital, National University Health System
Gabriel Yan: Division of Infectious Diseases, Department of Medicine, National University Hospital, National University Health System
Yong-Gui Gao: School of Biological Sciences, Nanyang Technological University
Meng How Tan: School of Chemical and Biomedical Engineering, Nanyang Technological University

DOI: https://doi.org/10.1038/s41467-021-21996-6
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 23

Abstract

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As the COVID-19 pandemic continues, variants of the virus are emerging. Here the authors present a diagnostic assay that can detect wildtype and known variants using engineered Cas12a.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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