Anthelminthic Effect of Dichrostachys cinerea on Infectious L3 Larvae of Cyathostomes Resistant to Albendazol.

Abstract

In vitro anthelminthic effect of raw aqueous extracts from leaves and bark of Dichrostachys cinerea on infectious L3 larvae of Cyathostomes resistant to Albendazol was evaluated. An inhibition assay for larval hatching was made. Third-stage larvae (L3) were exposed to raw aqueous extracts from leaves (4 mg/ml), and bark (6.8 mg/ml) of Dichrostachys cinerea, for three hours, then hatching was induced. Observations were made every 10 min. Paired T-Student was used to determine the differences in per cent of hatched larvae between the control and the treatment groups. GraphPadPrism 5.0.0 was used in the study. Significant inhibition levels were observed in all the extracts (P < 0.05) during the process; whereas hatching in the control group was 100 %, for the leaf and bark extracts of D. cinerea were 24.05, and 12.56 %, respectively. The results suggest the use of D. cinerea as an alternative anthelminthic treatment to Albendazol-resistant Cyasthostome populations.

Keywords

• parasitic nematodes
• cyasthostomes
• hatching
• third-stage larvae