The influence of growth regulators and explant position on the growth and development of Mandevilla sanderi (Hemsl.) Woodson in vitro

Abstract

Mandevilla is a valuable ornamental pot vine. However, due to a low propagation rate, it is difficult to keep up with the demand. Micropropagation would allow to produce lots of plants for the market. The aim of the study was to determine the effect of the growth regulators addition to the media and explants orientation on multiplication of Mandevilla sanderi, an exotic, ornamental pot plant. The shoot tips were placed vertically or horizontally on the Murashige and Skoog medium supplemented with benzyladenine (BA) or isopentenyladenine (2iP), at concentrations of 1, 2.5 or 5 mg·dm–3 singly or in combination with thidiazuron (TDZ) at concentrations of 0.01, 0.025 or 0.05 mg·dm–3. Maximum multiplication rate was noted on the media supplemented with 2.5 mg·dm–3 2iP + 0.025 mg·dm–3 TDZ or 5 mg·dm–3 2iP, when explants were placed horizontally. All the treatments resulted in callus formation. Medium supplemented with the highest concentration of BA combined with TDZ was the most active in callus growth.

Keywords

• Dipladenia
• Rocktrumpet
• micropropagation
• cytokinins
• explant orientation