An achievement has been made in establishing an induced pluripotent stem cell line (SDPHi005-A) from a healthy Chinese male donor

Jing Shao; Yan Jin; Chunhong Shao; Xiaoqiao Zhang; Guang Yang

Stem Cell Research (Jun 2024)

An achievement has been made in establishing an induced pluripotent stem cell line (SDPHi005-A) from a healthy Chinese male donor

Jing Shao,
Yan Jin,
Chunhong Shao,
Xiaoqiao Zhang,
Guang Yang

Affiliations

Jing Shao: Department of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250021, China
Yan Jin: Department of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250021, China
Chunhong Shao: Department of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250021, China
Xiaoqiao Zhang: Department of Gastrointestinal Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250021, China
Guang Yang: Department of General Surgery, The 960th Hospital of the PLA Joint Logistics Support Force, Jinan, Shandong 250031 China; Corresponding author.

Journal volume & issue: Vol. 77
p. 103393

Abstract

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Successfully transforming isolated peripheral blood mononuclear cells (PBMCs) from a healthy male into induced pluripotent stem cells (iPSCs) was achieved using non-integrating episomal vectors containing OCT4, SOX2, c-MYC, KLF4, and BCL-XL. The iPSCs exhibited a normal karyotype, expressed pluripotency markers, and demonstrated the ability to differentiate into three germ layers in vitro. This iPSC line may be employed for subsequent research purposes.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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