Generation of an induced pluripotent stem cell (iPSC) line from a patient with maturity-onset diabetes of the young type 3 (MODY3) carrying a hepatocyte nuclear factor 1-alpha (HNF1A) mutation

Frank Griscelli; Hélène Ezanno; Mathis Soubeyrand; Olivier Feraud; Noufissa Oudrhiri; Amélie Bonnefond; Ali G. Turhan; Philippe Froguel; Annelise Bennaceur-Griscelli

Stem Cell Research (May 2018)

Generation of an induced pluripotent stem cell (iPSC) line from a patient with maturity-onset diabetes of the young type 3 (MODY3) carrying a hepatocyte nuclear factor 1-alpha (HNF1A) mutation

Frank Griscelli,
Hélène Ezanno,
Mathis Soubeyrand,
Olivier Feraud,
Noufissa Oudrhiri,
Amélie Bonnefond,
Ali G. Turhan,
Philippe Froguel,
Annelise Bennaceur-Griscelli

Affiliations

Frank Griscelli: ESTeam Paris Sud, INGESTEM National IPSC Infrastructure, UPSud, Paris-Saclay University, Villejuif 94800, France; UMR-S 935, INSERM, University Paris Sud, Villejuif 94800, France; Gustave Roussy Institut, Department of Biopathology, Villejuif 94800, France; Paris Descartes University, Faculty Sorbonne Paris Cité, Faculté des Sciences Pharmaceutiques et Biologiques, Paris, France; Corresponding author at: ESTeam Paris Sud, INGESTEM National IPSC Infrastructure, UPSud, Paris-Saclay University, Villejuif 94800, France.
Hélène Ezanno: CNRS-UMR8199, Institut Pasteur de Lille, Lille, France; Lille University, Lille, France
Mathis Soubeyrand: ESTeam Paris Sud, INGESTEM National IPSC Infrastructure, UPSud, Paris-Saclay University, Villejuif 94800, France; UMR-S 935, INSERM, University Paris Sud, Villejuif 94800, France
Olivier Feraud: ESTeam Paris Sud, INGESTEM National IPSC Infrastructure, UPSud, Paris-Saclay University, Villejuif 94800, France; UMR-S 935, INSERM, University Paris Sud, Villejuif 94800, France
Noufissa Oudrhiri: ESTeam Paris Sud, INGESTEM National IPSC Infrastructure, UPSud, Paris-Saclay University, Villejuif 94800, France; UMR-S 935, INSERM, University Paris Sud, Villejuif 94800, France
Amélie Bonnefond: CNRS-UMR8199, Institut Pasteur de Lille, Lille, France; Lille University, Lille, France
Ali G. Turhan: ESTeam Paris Sud, INGESTEM National IPSC Infrastructure, UPSud, Paris-Saclay University, Villejuif 94800, France; UMR-S 935, INSERM, University Paris Sud, Villejuif 94800, France; Division of Hematology, APHP-Paris Sud University, Le Kremlin Bicêtre 94275, Villejuif 94800, France; Paris Sud University, UPsud, Faculty of Medicine, Le Kremlin Bicêtre 94275, France
Philippe Froguel: CNRS-UMR8199, Institut Pasteur de Lille, Lille, France; Lille University, Lille, France; Department of Genomics of Common Disease, School of Public Health, Imperial College London, Hammersmith Hospital, London, United Kingdom
Annelise Bennaceur-Griscelli: ESTeam Paris Sud, INGESTEM National IPSC Infrastructure, UPSud, Paris-Saclay University, Villejuif 94800, France; UMR-S 935, INSERM, University Paris Sud, Villejuif 94800, France; Division of Hematology, APHP-Paris Sud University, Le Kremlin Bicêtre 94275, Villejuif 94800, France; Paris Sud University, UPsud, Faculty of Medicine, Le Kremlin Bicêtre 94275, France

Journal volume & issue: Vol. 29
pp. 56 – 59

Abstract

Read online

Heterozygous non-synonymous (p.S142F) mutation in HNF1A leads to maturity-onset diabetes of the young (MODY) type 3, which is a subtype of dominant inherited young-onset non-autoimmune diabetes due to the defect of insulin secretion from pancreatic beta cells. We generated induced pluripotent stem cells (iPSCs) from a patient with HNF1A p.S142F mutation. Cells from this patient, which were reprogrammed by non-integrative viral transduction had normal karyotype, harboured the HNF1A p.S142F mutation, expressed pluripotency hallmarks.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

About the journal