In Vivo Characterization of the Anti-Glutathione S-Transferase Antibody Using an In Vitro Mite Feeding Model

Shwe Yee Win; Hikari Seo; Fumiya Horio; Sotaro Fujisawa; Jumpei Sato; Yoshinosuke Motai; Takumi Sato; Eiji Oishi; Akira Taneno; Lat Lat Htun; Saw Bawm; Tomohiro Okagawa; Naoya Maekawa; Satoru Konnai; Kazuhiko Ohashi; Shiro Murata

doi:10.3390/vaccines12020148

Vaccines (Jan 2024)

In Vivo Characterization of the Anti-Glutathione S-Transferase Antibody Using an In Vitro Mite Feeding Model

Shwe Yee Win,
Hikari Seo,
Fumiya Horio,
Sotaro Fujisawa,
Jumpei Sato,
Yoshinosuke Motai,
Takumi Sato,
Eiji Oishi,
Akira Taneno,
Lat Lat Htun,
Saw Bawm,
Tomohiro Okagawa,
Naoya Maekawa,
Satoru Konnai,
Kazuhiko Ohashi,
Shiro Murata

Affiliations

Shwe Yee Win: Laboratory of Infectious Diseases, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Kita-18, Nishi-9, Kita-ku, Sapporo 060-0818, Japan
Hikari Seo: Laboratory of Infectious Diseases, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Kita-18, Nishi-9, Kita-ku, Sapporo 060-0818, Japan
Fumiya Horio: Laboratory of Infectious Diseases, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Kita-18, Nishi-9, Kita-ku, Sapporo 060-0818, Japan
Sotaro Fujisawa: Laboratory of Infectious Diseases, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Kita-18, Nishi-9, Kita-ku, Sapporo 060-0818, Japan
Jumpei Sato: Laboratory of Infectious Diseases, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Kita-18, Nishi-9, Kita-ku, Sapporo 060-0818, Japan
Yoshinosuke Motai: Laboratory of Infectious Diseases, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Kita-18, Nishi-9, Kita-ku, Sapporo 060-0818, Japan
Takumi Sato: Vaxxinova Japan K.K., 1-24-8 Hamamatsucho, Minato-ku, Tokyo 105-0013, Japan
Eiji Oishi: Vaxxinova Japan K.K., 1-24-8 Hamamatsucho, Minato-ku, Tokyo 105-0013, Japan
Akira Taneno: Vaxxinova Japan K.K., 1-24-8 Hamamatsucho, Minato-ku, Tokyo 105-0013, Japan
Lat Lat Htun: Department of Pharmacology and Parasitology, University of Veterinary Science, Yezin, Nay Pyi Taw 15013, Myanmar
Saw Bawm: Department of Pharmacology and Parasitology, University of Veterinary Science, Yezin, Nay Pyi Taw 15013, Myanmar
Tomohiro Okagawa: Department of Advanced Pharmaceutics, Faculty of Veterinary Medicine, Hokkaido University, Kita-18, Nishi-9, Kita-ku, Sapporo 060-0818, Japan
Naoya Maekawa: Department of Advanced Pharmaceutics, Faculty of Veterinary Medicine, Hokkaido University, Kita-18, Nishi-9, Kita-ku, Sapporo 060-0818, Japan
Satoru Konnai: Laboratory of Infectious Diseases, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Kita-18, Nishi-9, Kita-ku, Sapporo 060-0818, Japan
Kazuhiko Ohashi: Laboratory of Infectious Diseases, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Kita-18, Nishi-9, Kita-ku, Sapporo 060-0818, Japan
Shiro Murata: Laboratory of Infectious Diseases, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Kita-18, Nishi-9, Kita-ku, Sapporo 060-0818, Japan

DOI: https://doi.org/10.3390/vaccines12020148
Journal volume & issue: Vol. 12, no. 2
p. 148

Abstract

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Poultry red mites (Dermanyssus gallinae, PRMs), tropical fowl mites (Ornithonyssus bursa, TFMs), and northern fowl mites (O. sylviarum, NFMs) are blood-feeding pests that debilitate poultry worldwide. Glutathione S-transferase (GST) plays an important role in the detoxification and drug metabolism of mites. However, research on avian mite GSTs as vaccine antigens is still lacking. Therefore, we aimed to evaluate the potential of avian mite GSTs for vaccine development. We identified GST genes from TFMs and NFMs. We prepared recombinant GST (rGST) from TFMs, NFMs, and PRMs, and assessed their protein functions. Moreover, we evaluated the cross-reactivity and acaricidal effect of immune plasma against each rGST on TFMs, NFMs, and PRMs. The deduced amino acid sequences of GSTs from TFMs and NFMs were 80% similar to those of the PRMs. The rGSTs exhibited catalytic activity in conjugating glutathione to the 1-chloro-2,4-dinitrobenzene substrate. Immune plasma against each rGST showed cross-reactivity with rGST from different mite species. Moreover, the survival rate of PRMs fed with immune plasma against the rGST of TFMs and NFMs was significantly lower than that of the control plasma. These results demonstrate the potential application of GST as an antigen for the development of a broad-spectrum vaccine against avian mites.

Published in Vaccines

ISSN: 2076-393X (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Medicine
Website: http://www.mdpi.com/journal/vaccines

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