Kualitas Spermatozoa Tikus Jantan yang Diberi Tepung Kedelai Kaya Isoflavon

Abstract

Reactive oxygen species (ROS) are free radicals that play a significant role in many of the sperm physiological processes. Spermatozoa need low amounts of endogenous ROS to inducing that processes for acquisition of spermfertilizing ability. Isoflavone as the active compound in soybean can act as scavenger free radicals, prevent formation of lipid peroxide, and prevent of oxidative stress condition on the tissue that produce spermatozoa. Twenty five males of Sprague Dawley weaning rats (21 days old) were divided into five groups and treated with isoflavone-riched soybean flour by oral administration with different levels (dosage) for 2 months. Conception rate and the number of fetus were evaluated on the mated-female rats. Diet was given as isonitrogen and isocaloric with 10% of dietary protein from casein for male and female rats. Result indicated that the treatment didn't affect significantly on spermatozoa abnormality. The treatment of isoflavone-riched soybean flour on male rats with highest dosage (6 mg isoflavone/day) resulted in infertility. The optimum dosage of isoflavone was 1.5 mg/day and resulted in increase of relative of weight testes, higher motility rate and sperm concentration of rat testes. Conclusion : Isoflavone-riched soybean flour treatment with 1.5 mg/day dosage of isoflavone improved the quality of spermatozoa of male rats.

Keywords

• Isoflavone-riched soybean flour
• quality of spermatozoa