Antibody toolkit reveals N-terminally ubiquitinated substrates of UBE2W

Christopher W. Davies; Simon E. Vidal; Lilian Phu; Jawahar Sudhamsu; Trent B. Hinkle; Scott Chan Rosenberg; Frances-Rose Schumacher; Yi Jimmy Zeng; Carsten Schwerdtfeger; Andrew S. Peterson; Jennie R. Lill; Christopher M. Rose; Andrey S. Shaw; Ingrid E. Wertz; Donald S. Kirkpatrick; James T. Koerber

doi:10.1038/s41467-021-24669-6

Nature Communications (Jul 2021)

Antibody toolkit reveals N-terminally ubiquitinated substrates of UBE2W

Christopher W. Davies,
Simon E. Vidal,
Lilian Phu,
Jawahar Sudhamsu,
Trent B. Hinkle,
Scott Chan Rosenberg,
Frances-Rose Schumacher,
Yi Jimmy Zeng,
Carsten Schwerdtfeger,
Andrew S. Peterson,
Jennie R. Lill,
Christopher M. Rose,
Andrey S. Shaw,
Ingrid E. Wertz,
Donald S. Kirkpatrick,
James T. Koerber

Affiliations

Christopher W. Davies: Department of Antibody Engineering, Genentech, Inc.
Simon E. Vidal: Departments of Molecular Oncology and Early Discovery Biochemistry, Genentech, Inc.
Lilian Phu: Department of Microchemistry, Proteomics, and Lipidomics, Genentech, Inc.
Jawahar Sudhamsu: Department of Structural Biology, Genentech, Inc.
Trent B. Hinkle: Department of Microchemistry, Proteomics, and Lipidomics, Genentech, Inc.
Scott Chan Rosenberg: Departments of Molecular Oncology and Early Discovery Biochemistry, Genentech, Inc.
Frances-Rose Schumacher: Department of Microchemistry, Proteomics, and Lipidomics, Genentech, Inc.
Yi Jimmy Zeng: Department of Microchemistry, Proteomics, and Lipidomics, Genentech, Inc.
Carsten Schwerdtfeger: Boston Biochem, a Bio-Techne Brand 840 Memorial Drive
Andrew S. Peterson: Department of Molecular Biology, Genentech, Inc.
Jennie R. Lill: Department of Microchemistry, Proteomics, and Lipidomics, Genentech, Inc.
Christopher M. Rose: Department of Microchemistry, Proteomics, and Lipidomics, Genentech, Inc.
Andrey S. Shaw: Research Biology, Genentech, Inc.
Ingrid E. Wertz: Departments of Molecular Oncology and Early Discovery Biochemistry, Genentech, Inc.
Donald S. Kirkpatrick: Department of Microchemistry, Proteomics, and Lipidomics, Genentech, Inc.
James T. Koerber: Department of Antibody Engineering, Genentech, Inc.

DOI: https://doi.org/10.1038/s41467-021-24669-6
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 19

Abstract

Read online

UBE2W catalyzes the ubiquitination of protein N-termini but its substrate spectrum is largely unknown. Here, the authors discover mAbs selective for peptides derived from N-terminally ubiquitinated proteins, solve the structure of a peptide-bound mAb and apply the mAbs to map endogenous UBE2W substrates by proteomics.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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