Utility of hybrid whole genome sequencing in assessing potential nosocomial VIM transmission

David Burke James Mahoney; Xena Li; Patryk Aftanas; Christie Vermeiren; Robert Kozak; Kevin Katz; Finlay Maguire

doi:10.1017/ash.2024.347

Antimicrobial Stewardship & Healthcare Epidemiology (Jan 2024)

Utility of hybrid whole genome sequencing in assessing potential nosocomial VIM transmission

David Burke James Mahoney,
Xena Li,
Patryk Aftanas,
Christie Vermeiren,
Robert Kozak,
Kevin Katz,
Finlay Maguire

Affiliations

David Burke James Mahoney: ORCiD; Department of Community Health and Epidemiology, Faculty of Medicine, Dalhousie University, Halifax, NS, Canada Faculty of Computer Science, Dalhousie University, Halifax, NS, Canada
Xena Li: ORCiD; Shared Hospital Laboratory, Toronto, ON, Canada Sunnybrook Health Sciences Centre, Toronto, ON, Canada Infection Prevention and Control, North York General Hospital, Toronto, ON, Canada
Patryk Aftanas: ORCiD; Shared Hospital Laboratory, Toronto, ON, Canada
Christie Vermeiren: Shared Hospital Laboratory, Toronto, ON, Canada Sunnybrook Health Sciences Centre, Toronto, ON, Canada Department of Laboratory Medicine and Pathology, University of Toronto, Toronto, ON, Canada
Robert Kozak: Shared Hospital Laboratory, Toronto, ON, Canada Sunnybrook Health Sciences Centre, Toronto, ON, Canada Department of Laboratory Medicine and Pathology, University of Toronto, Toronto, ON, Canada
Kevin Katz: Shared Hospital Laboratory, Toronto, ON, Canada Sunnybrook Health Sciences Centre, Toronto, ON, Canada Infection Prevention and Control, North York General Hospital, Toronto, ON, Canada Department of Laboratory Medicine and Pathology, University of Toronto, Toronto, ON, Canada
Finlay Maguire: ORCiD; Department of Community Health and Epidemiology, Faculty of Medicine, Dalhousie University, Halifax, NS, Canada Faculty of Computer Science, Dalhousie University, Halifax, NS, Canada Shared Hospital Laboratory, Toronto, ON, Canada Sunnybrook Health Sciences Centre, Toronto, ON, Canada

DOI: https://doi.org/10.1017/ash.2024.347
Journal volume & issue: Vol. 4

Abstract

Read online

Hybrid whole genome sequencing was used to investigate if nosocomial Verona integron-encoded metallo-β-lactamase (VIM) carbapenemase transmission occurred between two patients without epidemiological links or common pathogens. Challenges in genomic methodology and appropriate analytical depth for mobile carbapenemase outbreaks are described including how inappropriate choices can mislead results and impact infection control practices.

Published in Antimicrobial Stewardship & Healthcare Epidemiology

ISSN: 2732-494X (Online)
Publisher: Cambridge University Press
Country of publisher: United Kingdom
LCC subjects: Medicine: Internal medicine: Infectious and parasitic diseases; Medicine: Public aspects of medicine
Website: https://www.cambridge.org/core/journals/antimicrobial-stewardship-and-healthcare-epidemiology

About the journal