Bioactive compounds, pigment content and antioxidant activity of pouzolzia zeylanica plant collected at different growth stages

Abstract

The aim of this study was to investigative the effect of different growth stages of Pouzolzia zeylanica plant (young and mature plant) on bioactive compounds content (anthocyanin, flavonoid, polyphenol and tannin); the content of pigments (chlorophyll a, chlorophyll b, total chlorophyll and carotenoids); antioxidant activity through some measurable values such as antioxidant ability index (AAI), ferrous reducing ability power (FRAP) and scavenging capacity 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical; as well as the color parameters of stem and leaves (L*, a*, b* and DE). The results showed that the content of anthocyanin, flavonoid, polyphenol, chlorophyll a, chlorophyll b, total chlorophyll and carotenoids of young plant was higher than that of mature ones, and had statistically significant difference (P0.05). There was no difference in antioxidant capacity between young and mature plants when performed with scavenging free radical (DPPH) or total reducing power (AAI), but there was significant difference when performed with ferrous reducing ability power (FRAP) method. In addition, there were also statistically significant differences in average values of a* and b* between young and mature, stem and leaves, and these two parameters were related to the content of anthocyanin, chlorophyll, carotenoid in Pouzolzia zeylanica plant.

Keywords

• Antioxidant activity
• bioactive compounds
• growth stage
• pigments
• Pouzolzia zeylanica