RNF219 attenuates global mRNA decay through inhibition of CCR4-NOT complex-mediated deadenylation

Fabian Poetz; Joshua Corbo; Yevgen Levdansky; Alexander Spiegelhalter; Doris Lindner; Vera Magg; Svetlana Lebedeva; Jörg Schweiggert; Johanna Schott; Eugene Valkov; Georg Stoecklin

doi:10.1038/s41467-021-27471-6

Nature Communications (Dec 2021)

RNF219 attenuates global mRNA decay through inhibition of CCR4-NOT complex-mediated deadenylation

Fabian Poetz,
Joshua Corbo,
Yevgen Levdansky,
Alexander Spiegelhalter,
Doris Lindner,
Vera Magg,
Svetlana Lebedeva,
Jörg Schweiggert,
Johanna Schott,
Eugene Valkov,
Georg Stoecklin

Affiliations

Fabian Poetz: Division of Biochemistry, Mannheim Institute for Innate Immunoscience (MI3), Medical Faculty Mannheim, Heidelberg University
Joshua Corbo: RNA Biology Laboratory, Center for Cancer Research, National Cancer Institute (NCI)
Yevgen Levdansky: RNA Biology Laboratory, Center for Cancer Research, National Cancer Institute (NCI)
Alexander Spiegelhalter: Division of Biochemistry, Mannheim Institute for Innate Immunoscience (MI3), Medical Faculty Mannheim, Heidelberg University
Doris Lindner: Division of Biochemistry, Mannheim Institute for Innate Immunoscience (MI3), Medical Faculty Mannheim, Heidelberg University
Vera Magg: Department of Infectious Diseases, Molecular Virology, Center for Integrative Infectious Disease Research (CIID), Heidelberg University
Svetlana Lebedeva: Berlin Institute for Molecular Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine
Jörg Schweiggert: Center for Molecular Biology of Heidelberg University (ZMBH), German Cancer Research Center (DKFZ)-ZMBH Alliance
Johanna Schott: Division of Biochemistry, Mannheim Institute for Innate Immunoscience (MI3), Medical Faculty Mannheim, Heidelberg University
Eugene Valkov: RNA Biology Laboratory, Center for Cancer Research, National Cancer Institute (NCI)
Georg Stoecklin: Division of Biochemistry, Mannheim Institute for Innate Immunoscience (MI3), Medical Faculty Mannheim, Heidelberg University

DOI: https://doi.org/10.1038/s41467-021-27471-6
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 19

Abstract

Read online

The CCR4-NOT complex is a key regulator of eukaryotic mRNA deadenylation. Here the authors identified the E3 ubiquitin ligase RNF219 as an acetylation-regulated cofactor of the complex, which inhibits mRNA decay through a direct interaction with NOT9.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal