Possibilidades e limitações no uso da temperatura em cromatografia líquida de fase reversa Possibilities and limitations of using temperature in reversed phase liquid chromatography

Abstract

High-temperature liquid chromatography (HTLC) is a technique that presents a series of advantages in liquid phase separations, such as: reduced analysis time, reduced pressure drop, reduced asymmetry factors, modified retentions, controlled selectivities, better efficiencies and improved detectivities, as well as permitting green chromatography. The practical limitations that relate to instrumentation and to stationary phase instability are being resolved and this technique is now ready to be applied for routine determinations.

Keywords

• high temperature liquid chromatography
• stationary phase stability
• green chromatography