Frontiers in Molecular Biosciences (Nov 2022)
The rational design of iron-sulfur cluster binding site for prolonged stability in magnetoreceptor MagR
- Tianyang Tong,
- Tianyang Tong,
- Yajie Zhou,
- Yajie Zhou,
- Fan Fei,
- Fan Fei,
- Xiujuan Zhou,
- Xiujuan Zhou,
- Zhen Guo,
- Shun Wang,
- Shun Wang,
- Jing Zhang,
- Jing Zhang,
- Peng Zhang,
- Peng Zhang,
- Tiantian Cai,
- Guohui Li,
- Yuebin Zhang,
- Junfeng Wang,
- Junfeng Wang,
- Junfeng Wang,
- Junfeng Wang,
- Can Xie,
- Can Xie,
- Can Xie,
- Can Xie
Affiliations
- Tianyang Tong
- Department of Anatomy, School of Basic Medicine, Anhui Medical University, Hefei, Anhui, China
- Tianyang Tong
- High Magnetic Field Laboratory, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Science Island, Hefei, China
- Yajie Zhou
- High Magnetic Field Laboratory, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Science Island, Hefei, China
- Yajie Zhou
- Institutes of Physical Science and Information Technology, Anhui University, Hefei, Anhui, China
- Fan Fei
- High Magnetic Field Laboratory, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Science Island, Hefei, China
- Fan Fei
- Science Island Branch of Graduate School, University of Science and Technology of China, Hefei, Anhui, China
- Xiujuan Zhou
- High Magnetic Field Laboratory, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Science Island, Hefei, China
- Xiujuan Zhou
- Science Island Branch of Graduate School, University of Science and Technology of China, Hefei, Anhui, China
- Zhen Guo
- School of Life Sciences, Peking University, Beijing, China
- Shun Wang
- High Magnetic Field Laboratory, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Science Island, Hefei, China
- Shun Wang
- Institutes of Physical Science and Information Technology, Anhui University, Hefei, Anhui, China
- Jing Zhang
- High Magnetic Field Laboratory, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Science Island, Hefei, China
- Jing Zhang
- Science Island Branch of Graduate School, University of Science and Technology of China, Hefei, Anhui, China
- Peng Zhang
- High Magnetic Field Laboratory, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Science Island, Hefei, China
- Peng Zhang
- Science Island Branch of Graduate School, University of Science and Technology of China, Hefei, Anhui, China
- Tiantian Cai
- Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, United States
- Guohui Li
- State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China
- Yuebin Zhang
- State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China
- Junfeng Wang
- Department of Anatomy, School of Basic Medicine, Anhui Medical University, Hefei, Anhui, China
- Junfeng Wang
- High Magnetic Field Laboratory, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Science Island, Hefei, China
- Junfeng Wang
- Science Island Branch of Graduate School, University of Science and Technology of China, Hefei, Anhui, China
- Junfeng Wang
- International Magnetobiology Frontier Research Center, Science Island, Hefei, China
- Can Xie
- Department of Anatomy, School of Basic Medicine, Anhui Medical University, Hefei, Anhui, China
- Can Xie
- High Magnetic Field Laboratory, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Science Island, Hefei, China
- Can Xie
- Science Island Branch of Graduate School, University of Science and Technology of China, Hefei, Anhui, China
- Can Xie
- International Magnetobiology Frontier Research Center, Science Island, Hefei, China
- DOI
- https://doi.org/10.3389/fmolb.2022.1051943
- Journal volume & issue
-
Vol. 9
Abstract
Iron-sulfur proteins play essential roles in a wide variety of cellular processes such as respiration, photosynthesis, nitrogen fixation and magnetoreception. The stability of iron-sulfur clusters varies significantly between anaerobic and aerobic conditions due to their intrinsic sensitivity to oxygen. Iron-sulfur proteins are well suited to various practical applications as molecular redox sensors or molecular “wires” for electron transfer. Various technologies have been developed recently using one particular iron-sulfur protein, MagR, as a magnetic tag. However, the limited protein stability and low magnetic sensitivity of MagR hindered its wide application. Here in this study, the iron-sulfur binding site of pigeon clMagR was rationally re-designed. One such mutation, T57C in pigeon MagR, showed improved iron-sulfur binding efficiency and higher iron content, as well as prolonged thermostability. Thus, clMagRT57C can serve as a prototype for further design of more stable and sensitive magnetic toolbox for magnetogenetics in the future.
Keywords
