Nature Communications (Oct 2022)

A universal glycoenzyme biosynthesis pipeline that enables efficient cell-free remodeling of glycans

  • Thapakorn Jaroentomeechai,
  • Yong Hyun Kwon,
  • Yiwen Liu,
  • Olivia Young,
  • Ruchika Bhawal,
  • Joshua D. Wilson,
  • Mingji Li,
  • Digantkumar G. Chapla,
  • Kelley W. Moremen,
  • Michael C. Jewett,
  • Dario Mizrachi,
  • Matthew P. DeLisa

DOI
https://doi.org/10.1038/s41467-022-34029-7
Journal volume & issue
Vol. 13, no. 1
pp. 1 – 17

Abstract

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Access to glycoenzymes for basic and applied research is limited by difficulties with their recombinant expression. Here, the authors describe a universal strategy for converting membrane-bound glycosyltransferases into water-soluble biocatalysts, which are expressed at high levels with retention of activity.