Frontiers in Veterinary Science (Sep 2024)
Wuzhishan miniature pig-derived intestinal 2D monolayer organoids to investigate the enteric coronavirus infection
- Yuanyuan Liu,
- Yuanyuan Liu,
- Yuanyuan Liu,
- Ning Yang,
- Ning Yang,
- Chen Tan,
- Chen Tan,
- Chen Tan,
- Yunhang Zhang,
- Yunhang Zhang,
- Yunhang Zhang,
- Shuai Gao,
- Yifei Cai,
- Yifei Cai,
- Yifei Cai,
- Yue Zhang,
- Yuguang Fu,
- Guangliang Liu,
- Guangliang Liu,
- Yang Li,
- Yang Li
Affiliations
- Yuanyuan Liu
- Key Laboratory of Tropical Animal Breeding and Disease Research, Institute of Animal Husbandry and Veterinary Medicine, Hainan Academy of Agricultural Sciences, Haikou, China
- Yuanyuan Liu
- State Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China
- Yuanyuan Liu
- College of Veterinary Medicine, Xinjiang Agricultural University, Ürümqi, China
- Ning Yang
- State Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China
- Ning Yang
- Molecular and Cellular Epigenetics (GIGA), University of Liège, Liège, Belgium
- Chen Tan
- Key Laboratory of Tropical Animal Breeding and Disease Research, Institute of Animal Husbandry and Veterinary Medicine, Hainan Academy of Agricultural Sciences, Haikou, China
- Chen Tan
- State Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China
- Chen Tan
- Molecular and Cellular Epigenetics (GIGA), University of Liège, Liège, Belgium
- Yunhang Zhang
- Key Laboratory of Tropical Animal Breeding and Disease Research, Institute of Animal Husbandry and Veterinary Medicine, Hainan Academy of Agricultural Sciences, Haikou, China
- Yunhang Zhang
- State Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China
- Yunhang Zhang
- Molecular and Cellular Epigenetics (GIGA), University of Liège, Liège, Belgium
- Shuai Gao
- Key Laboratory of Tropical Animal Breeding and Disease Research, Institute of Animal Husbandry and Veterinary Medicine, Hainan Academy of Agricultural Sciences, Haikou, China
- Yifei Cai
- Key Laboratory of Tropical Animal Breeding and Disease Research, Institute of Animal Husbandry and Veterinary Medicine, Hainan Academy of Agricultural Sciences, Haikou, China
- Yifei Cai
- State Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China
- Yifei Cai
- Human Nutrition and Health Group, VLAG, Wageningen University & Research, Wageningen, Netherlands
- Yue Zhang
- State Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China
- Yuguang Fu
- State Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China
- Guangliang Liu
- Key Laboratory of Tropical Animal Breeding and Disease Research, Institute of Animal Husbandry and Veterinary Medicine, Hainan Academy of Agricultural Sciences, Haikou, China
- Guangliang Liu
- State Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China
- Yang Li
- Key Laboratory of Tropical Animal Breeding and Disease Research, Institute of Animal Husbandry and Veterinary Medicine, Hainan Academy of Agricultural Sciences, Haikou, China
- Yang Li
- State Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China
- DOI
- https://doi.org/10.3389/fvets.2024.1457719
- Journal volume & issue
-
Vol. 11
Abstract
Intestinal organoids are valuable tools for investigating intestinal physiology and pathology ex vivo. In previous studies, intestinal organoids of commercial pigs have been developed. Here, we established intestinal organoids derived from Wuzhishan miniature pigs (WZS pigs), a unique kind of pig in the Hainan province of China. Three-dimensional (3D) intestinal organoids and organoid monolayers were developed and assessed. Furthermore, the susceptibility of organoid monolayers of WZS pigs to transmissible gastroenteritis virus (TGEV) was demonstrated. An RNA-seq analysis revealed that the TGEV infection stimulated antiviral and inflammatory immune responses in organoid monolayer models. The study implied the transmission risk of swine enteric coronavirus on WZS pigs and provided useful tools for further research on WZS pigs as laboratory miniature pig models.
Keywords
