YAP-depleted iPSC MUSIi012-A-2 maintained all normal stem cell characteristics

Chanchao Lorthongpanich; Chuti Laowtammathron; Nittaya Jiamvoraphong; Pimonwan Srisook; Pimjai Chingsuwanrote; Phatchanat Klaihmon; Nattaya Damkham; Papussorn Terbto; Supaporn Waeteekul; Yaowalak U-pratya; Surapol Issaragrisil

Stem Cell Research (Mar 2020)

YAP-depleted iPSC MUSIi012-A-2 maintained all normal stem cell characteristics

Chanchao Lorthongpanich,
Chuti Laowtammathron,
Nittaya Jiamvoraphong,
Pimonwan Srisook,
Pimjai Chingsuwanrote,
Phatchanat Klaihmon,
Nattaya Damkham,
Papussorn Terbto,
Supaporn Waeteekul,
Yaowalak U-pratya,
Surapol Issaragrisil

Affiliations

Chanchao Lorthongpanich: Siriraj Center of Excellence for Stem Cell Research, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand; Corresponding author.
Chuti Laowtammathron: Siriraj Center of Excellence for Stem Cell Research, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand
Nittaya Jiamvoraphong: Siriraj Center of Excellence for Stem Cell Research, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand
Pimonwan Srisook: Siriraj Center of Excellence for Stem Cell Research, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand
Pimjai Chingsuwanrote: Siriraj Center of Excellence for Stem Cell Research, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand
Phatchanat Klaihmon: Siriraj Center of Excellence for Stem Cell Research, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand
Nattaya Damkham: Siriraj Center of Excellence for Stem Cell Research, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand; Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
Papussorn Terbto: Department of Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
Supaporn Waeteekul: Division of Medical Genetics, Department of Obstetrics & Gynecology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
Yaowalak U-pratya: Siriraj Center of Excellence for Stem Cell Research, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand; Division of Hematology, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
Surapol Issaragrisil: Siriraj Center of Excellence for Stem Cell Research, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand; Division of Hematology, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; Bangkok Hematology Center, Wattanosoth Hospital, BDMS Center of Excellence for Cancer, Bangkok, Thailand

Journal volume & issue: Vol. 43

Abstract

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Yes-associated protein (YAP) is an important transcriptional coactivator in the Hippo signaling pathway. Using CRISPR/Cas9 technology, we established a stable YAP-knockdown (YAP-KD) induced pluripotent stem cell (iPSC) from the MUSIi012-A cell line. The YAP-KD iPSC MUSIi012-A-2 maintained the pluripotent phenotype, the ability to differentiate into all three embryonic germ layers, and it maintained the normal karyotype. Keywords: YAP, Hippo signaling pathway, iPSC

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

About the journal