Biofilm Production Ability, Virulence and Antimicrobial Resistance Genes in <i>Staphylococcus aureus</i> from Various Veterinary Hospitals

Lin Chen; Zi-Yun Tang; Shi-Yun Cui; Zhen-Bao Ma; Hua Deng; Wei-Li Kong; Li-Wen Yang; Chao Lin; Wen-Guang Xiong; Zhen-Ling Zeng

doi:10.3390/pathogens9040264

Pathogens (Apr 2020)

Biofilm Production Ability, Virulence and Antimicrobial Resistance Genes in <i>Staphylococcus aureus</i> from Various Veterinary Hospitals

Lin Chen,
Zi-Yun Tang,
Shi-Yun Cui,
Zhen-Bao Ma,
Hua Deng,
Wei-Li Kong,
Li-Wen Yang,
Chao Lin,
Wen-Guang Xiong,
Zhen-Ling Zeng

Affiliations

Lin Chen: Guangdong Provincial Key Laboratory of Veterinary Drugs Development and Safety Evaluation, National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou 510642, China
Zi-Yun Tang: Guangdong Provincial Key Laboratory of Veterinary Drugs Development and Safety Evaluation, National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou 510642, China
Shi-Yun Cui: Guangdong Provincial Key Laboratory of Veterinary Drugs Development and Safety Evaluation, National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou 510642, China
Zhen-Bao Ma: Guangdong Provincial Key Laboratory of Veterinary Drugs Development and Safety Evaluation, National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou 510642, China
Hua Deng: School of Life Science and Engineering, Foshan University, Foshan 528231, China
Wei-Li Kong: Guangdong Provincial Key Laboratory of Veterinary Drugs Development and Safety Evaluation, National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou 510642, China
Li-Wen Yang: Guangdong Provincial Key Laboratory of Veterinary Drugs Development and Safety Evaluation, National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou 510642, China
Chao Lin: Guangdong Provincial Key Laboratory of Veterinary Drugs Development and Safety Evaluation, National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou 510642, China
Wen-Guang Xiong: Guangdong Provincial Key Laboratory of Veterinary Drugs Development and Safety Evaluation, National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou 510642, China
Zhen-Ling Zeng: Guangdong Provincial Key Laboratory of Veterinary Drugs Development and Safety Evaluation, National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou 510642, China

DOI: https://doi.org/10.3390/pathogens9040264
Journal volume & issue: Vol. 9, no. 4
p. 264

Abstract

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Staphylococcus aureus (S. aureus) is one of the most clinically important zoonotic pathogens, but an understanding of the prevalence, biofilm formulation ability, virulence, and antimicrobial resistance genes of S. aureus from veterinary hospitals is lacking. By characterizing S. aureus in different origins of veterinary hospitals in Guangzhou, China, in 2019, we identified with the presence of S. aureus in pets (17.1%), veterinarians (31.7%), airborne dust (19.1%), environmental surfaces (4.3%), and medical device surfaces (10.8%). Multilocus sequence typing (MLST) and Staphylococcus protein A (spa) typing analyses demonstrated methicillin-sensitive S. aureus (MSSA) ST398-t571, MSSA ST188-t189, and methicillin-resistant S. aureus (MRSA) ST59-t437 were the most prevalent lineage. S. aureus with similar pulsed-field gel electrophoresis (PFGE) types distributed widely in different kinds of samples. The crystal violet straining assays revealed 100% (3/3) of MRSA ST59 and 81.8% (9/11) of MSSA ST188 showed strong biofilm formulation ability, whereas other STs (ST1, ST5, ST7, ST15, ST88, ST398, ST3154 and ST5353) showed weak biofilm production ability. Polymerase chain reaction (PCR) confirmed the most prevalent leucocidin, staphylococcal enterotoxins, ica operon, and adhesion genes were lukD-lukE (49.0%), sec-sel (15.7%), icaA-icaB-icaC-icaR (100.0%), and fnbB-cidA-fib-ebps-eno (100.0%), respectively. Our study showed that the isolates with strong biofilm production ability had a higher prevalence in clfA, clfB, fnbA and sdrC genes compared to the isolates with weak biofilm production ability. Furthermore, 2 ST1-MRSA isolates with tst gene and 1 ST88-MSSA isolate with lukS/F-PV gene were detected. In conclusion, the clonal dissemination of S. aureus of different origins in veterinary hospitals may have occurred; the biofilm production capacity of S. aureus is strongly correlated with ST types; some adhesion genes such as clfA, clfB, fnbA, and sdrC may pose an influence on biofilm production ability and the emergence of lukS/F-PV and tst genes in S. aureus from veterinary hospitals should raise our vigilance.

Published in Pathogens

ISSN: 2076-0817 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Medicine
Website: http://www.mdpi.com/journal/pathogens

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