Nature Communications (Feb 2023)
Sample multiplexing-based targeted pathway proteomics with real-time analytics reveals the impact of genetic variation on protein expression
- Qing Yu,
- Xinyue Liu,
- Mark P. Keller,
- Jose Navarrete-Perea,
- Tian Zhang,
- Sipei Fu,
- Laura P. Vaites,
- Steven R. Shuken,
- Ernst Schmid,
- Gregory R. Keele,
- Jiaming Li,
- Edward L. Huttlin,
- Edrees H. Rashan,
- Judith Simcox,
- Gary A. Churchill,
- Devin K. Schweppe,
- Alan D. Attie,
- Joao A. Paulo,
- Steven P. Gygi
Affiliations
- Qing Yu
- Department of Cell Biology, Harvard Medical School
- Xinyue Liu
- Department of Cell Biology, Harvard Medical School
- Mark P. Keller
- Department of Biochemistry, University of Wisconsin-Madison
- Jose Navarrete-Perea
- Department of Cell Biology, Harvard Medical School
- Tian Zhang
- Department of Cell Biology, Harvard Medical School
- Sipei Fu
- Department of Cell Biology, Harvard Medical School
- Laura P. Vaites
- Department of Cell Biology, Harvard Medical School
- Steven R. Shuken
- Department of Cell Biology, Harvard Medical School
- Ernst Schmid
- Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School
- Gregory R. Keele
- The Jackson Laboratory
- Jiaming Li
- Department of Cell Biology, Harvard Medical School
- Edward L. Huttlin
- Department of Cell Biology, Harvard Medical School
- Edrees H. Rashan
- Department of Biochemistry, University of Wisconsin-Madison
- Judith Simcox
- Department of Biochemistry, University of Wisconsin-Madison
- Gary A. Churchill
- The Jackson Laboratory
- Devin K. Schweppe
- Department of Genome Sciences, University of Washington
- Alan D. Attie
- Department of Biochemistry, University of Wisconsin-Madison
- Joao A. Paulo
- Department of Cell Biology, Harvard Medical School
- Steven P. Gygi
- Department of Cell Biology, Harvard Medical School
- DOI
- https://doi.org/10.1038/s41467-023-36269-7
- Journal volume & issue
-
Vol. 14,
no. 1
pp. 1 – 16
Abstract
Targeted proteomics enables robust hypothesis-driven research. Here, Yu et al. present a multiplexed approach for targeted pathway proteomics and apply it to quantify protein families across 480 fully genotyped Diversity Outbred mice, revealing impacts of genetic variation on protein expression and lipid metabolism.
