Communications Biology (Dec 2022)

Protein structure determination in human cells by in-cell NMR and a reporter system to optimize protein delivery or transexpression

  • Juan A. Gerez,
  • Natalia C. Prymaczok,
  • Harindranath Kadavath,
  • Dhiman Ghosh,
  • Matthias Bütikofer,
  • Yanick Fleischmann,
  • Peter Güntert,
  • Roland Riek

DOI
https://doi.org/10.1038/s42003-022-04251-6
Journal volume & issue
Vol. 5, no. 1
pp. 1 – 14

Abstract

Read online

An improved method for in-cell NMR is developed using a reporter to evaluate protein delivery and delivery parameters to different cell lines and applied to IDPs and folded proteins including structure determination of GB1 at 1.1 Å resolution.