Transcriptome Analysis of Response to Aluminum Stress in <i>Pinus massoniana</i>

Abstract

Pinus massoniana is a vital kind of coniferous species rich in rosin. Aluminum stress is a severe problem for P. massoniana growth in acidic soil causing root poisoning. However, the molecular mechanisms of aluminum-responsive are still unclear. We performed a transcriptome analysis of the P. massoniana root in response to aluminum stress. Through WGCNA analysis, we identified 338 early and 743 late response genes to aluminum stress. Gene Ontology analysis found many critical functional pathways, such as carbohydrate binding, cellulase activity, and phenylalanine ammonia-lyase activity. In addition, KEGG analysis revealed a significant enrichment of phenylpropanoid biosynthesis pathways. Further analysis showed that the expression of lignin synthesis genes 4CL, CAD, and COMT were up-regulated, indicating that they may play a crucial role in the process of aluminum tolerance in P. massoniana roots. These results provide method support for studying the regulation mechanism of P. massoniana aluminum stress.

Keywords

• <i>Pinus massoniana</i>
• aluminum stress
• transcriptomic
• WGCNA analysis
• phenylpropanoid biosynthesis