TRACE: An Unbiased Method to Permanently Tag Transiently Activated Inputs

Nathalie Krauth; Nathalie Krauth; Valentina Khalil; Valentina Khalil; Meet Jariwala; Meet Jariwala; Noemie Mermet-Joret; Noemie Mermet-Joret; Anne-Katrine Vestergaard; Anne-Katrine Vestergaard; Marco Capogna; Marco Capogna; Marco Capogna; Sadegh Nabavi; Sadegh Nabavi; Sadegh Nabavi

doi:10.3389/fncel.2020.00114

Frontiers in Cellular Neuroscience (May 2020)

TRACE: An Unbiased Method to Permanently Tag Transiently Activated Inputs

Nathalie Krauth,
Nathalie Krauth,
Valentina Khalil,
Valentina Khalil,
Meet Jariwala,
Meet Jariwala,
Noemie Mermet-Joret,
Noemie Mermet-Joret,
Anne-Katrine Vestergaard,
Anne-Katrine Vestergaard,
Marco Capogna,
Marco Capogna,
Marco Capogna,
Sadegh Nabavi,
Sadegh Nabavi,
Sadegh Nabavi

Affiliations

Nathalie Krauth: Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark
Nathalie Krauth: DANDRITE, The Danish Research Institute of Translational Neuroscience, Aarhus, Denmark
Valentina Khalil: Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark
Valentina Khalil: DANDRITE, The Danish Research Institute of Translational Neuroscience, Aarhus, Denmark
Meet Jariwala: Department of Biomedicine, Aarhus University, Aarhus, Denmark
Meet Jariwala: Center for Proteins in Memory—PROMEMO, Danish National Research Foundation, Aarhus, Denmark
Noemie Mermet-Joret: Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark
Noemie Mermet-Joret: DANDRITE, The Danish Research Institute of Translational Neuroscience, Aarhus, Denmark
Anne-Katrine Vestergaard: Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark
Anne-Katrine Vestergaard: DANDRITE, The Danish Research Institute of Translational Neuroscience, Aarhus, Denmark
Marco Capogna: DANDRITE, The Danish Research Institute of Translational Neuroscience, Aarhus, Denmark
Marco Capogna: Department of Biomedicine, Aarhus University, Aarhus, Denmark
Marco Capogna: Center for Proteins in Memory—PROMEMO, Danish National Research Foundation, Aarhus, Denmark
Sadegh Nabavi: Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark
Sadegh Nabavi: DANDRITE, The Danish Research Institute of Translational Neuroscience, Aarhus, Denmark
Sadegh Nabavi: Center for Proteins in Memory—PROMEMO, Danish National Research Foundation, Aarhus, Denmark

DOI: https://doi.org/10.3389/fncel.2020.00114
Journal volume & issue: Vol. 14

Abstract

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A fundamental interest in circuit analysis is to parse out the synaptic inputs underlying a behavioral experience. Toward this aim, we have devised an unbiased strategy that specifically labels the afferent inputs that are activated by a defined stimulus in an activity-dependent manner. We validated this strategy in four brain circuits receiving known sensory inputs. This strategy, as demonstrated here, accurately identifies these inputs.

Published in Frontiers in Cellular Neuroscience

ISSN: 1662-5102 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Medicine: Internal medicine: Neurosciences. Biological psychiatry. Neuropsychiatry
Website: http://www.frontiersin.org/cellular-neuroscience

About the journal

Abstract

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