International Journal of Nanomedicine (Mar 2020)
An Electrochemical Aptasensor Platform Based on Flower-Like Gold Microstructure-Modified Screen-Printed Carbon Electrode for Detection of Serpin A12 as a Type 2 Diabetes Biomarker
Abstract
Armin Salek Maghsoudi,1,* Shokoufeh Hassani,1,2,* Milad Rezaei Akmal,3 Mohammad Reza Ganjali,3,4 Kayvan Mirnia,5 Parviz Norouzi,3,4 Mohammad Abdollahi1,2 1Department of Toxicology and Pharmacology, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran; 2Toxicology and Diseases Group (TDG), Pharmaceutical Sciences Research Center (PSRC), The Institute of Pharmaceutical Sciences (TIPS), Tehran University of Medical Sciences, Tehran, Iran; 3Center of Excellence in Electrochemistry, Faculty of Chemistry, University of Tehran, Tehran, Iran; 4Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran; 5Department of Neonatology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran*These authors contributed equally to this workCorrespondence: Mohammad AbdollahiToxicology and Diseases Group (TDG), Pharmaceutical Sciences Research Center (PSRC), The Institute of Pharmaceutical Sciences (TIPS), Tehran University of Medical Sciences, Tehran 1417614411, IranTel +98 21 64122319Email [email protected]: In the present study, a highly sensitive and simple electrochemical (EC) aptasensor for the detection of serpin A12 as a novel biomarker of diabetes was developed on a platform where flower-like gold microstructures (FLGMs) are electrodeposited onto a disposable screen-printed carbon electrode. Meanwhile, serpin A12-specific thiolated aptamer was covalently immobilized on the FLGMs.Methods: The electrochemical activity of a fabricated aptasensor under various conditions were examined by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Aptamer concentration, deposition time, self-assembly time, and incubation time were optimized for assay of serpin A12. The differential pulse voltammetry (DPV) was implemented for quantitative detection of serpin A12 in K3 [Fe (CN) 6]/K4 [Fe (CN) 6] solution (redox probe).Results: The label-free aptasensor revealed a linear range of serpin A12 concentration (0.039– 10 ng/mL), detection limit of 0.020 ng/mL (S/N=3), and 0.031 ng/mL in solution buffer and plasma, respectively.Conclusion: The results indicate that this aptasensor has a high sensitivity, selectivity, stability, and acceptable reproducibility for detection of serpin A12 in diabetic patients.Keywords: electrochemical aptasensor, screen-printed carbon electrode, flower-like gold microstructures, label-free detection, serpin A12, diabetes biomarkers
