Development and Validation of an LC-MS/MS Method for Simultaneous Determination of Short Peptide-Based Drugs in Human Blood Plasma

Elizaveta N. Fisher; Evgeny S. Melnikov; Vladimir Gegeckori; Natalya V. Potoldykova; Dmitry V. Enikeev; Kirill A. Pavlenko; Snezana Agatonovic-Kustrin; David W. Morton; Galina V. Ramenskaya

doi:10.3390/molecules27227831

Molecules (Nov 2022)

Development and Validation of an LC-MS/MS Method for Simultaneous Determination of Short Peptide-Based Drugs in Human Blood Plasma

Elizaveta N. Fisher,
Evgeny S. Melnikov,
Vladimir Gegeckori,
Natalya V. Potoldykova,
Dmitry V. Enikeev,
Kirill A. Pavlenko,
Snezana Agatonovic-Kustrin,
David W. Morton,
Galina V. Ramenskaya

Affiliations

Elizaveta N. Fisher: I.M. Sechenov First MSMU of the Ministry of Health of the Russian Federation, Sechenov University, 8, Trubetskaya Street, 119991 Moscow, Russia
Evgeny S. Melnikov: I.M. Sechenov First MSMU of the Ministry of Health of the Russian Federation, Sechenov University, 8, Trubetskaya Street, 119991 Moscow, Russia
Vladimir Gegeckori: I.M. Sechenov First MSMU of the Ministry of Health of the Russian Federation, Sechenov University, 8, Trubetskaya Street, 119991 Moscow, Russia
Natalya V. Potoldykova: Institute for Urology and Reproductive Health, I.M. Sechenov First MSMU of the Ministry of Health of the Russian Federation, Sechenov University, 8, Trubetskaya Street, 119991 Moscow, Russia
Dmitry V. Enikeev: Institute for Urology and Reproductive Health, I.M. Sechenov First MSMU of the Ministry of Health of the Russian Federation, Sechenov University, 8, Trubetskaya Street, 119991 Moscow, Russia
Kirill A. Pavlenko: Moscow Clinical Scientific Center, 86, Shosse Enthuziastov, 111123 Moscow, Russia
Snezana Agatonovic-Kustrin: I.M. Sechenov First MSMU of the Ministry of Health of the Russian Federation, Sechenov University, 8, Trubetskaya Street, 119991 Moscow, Russia
David W. Morton: I.M. Sechenov First MSMU of the Ministry of Health of the Russian Federation, Sechenov University, 8, Trubetskaya Street, 119991 Moscow, Russia
Galina V. Ramenskaya: I.M. Sechenov First MSMU of the Ministry of Health of the Russian Federation, Sechenov University, 8, Trubetskaya Street, 119991 Moscow, Russia

DOI: https://doi.org/10.3390/molecules27227831
Journal volume & issue: Vol. 27, no. 22
p. 7831

Abstract

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A novel HPLC-ESI-MS/MS method for simultaneous gonadotropin-releasing hormone (GnRH) analogs and somatostatin analog quantitation was developed and validated. The developed method was successfully applied to pharmacokinetic studies. The sample preparation process included solid-phase extraction (SPE). Effective chromatographic separation of the analytes and internal standard (dalargin) was achieved with a C18 column, using a gradient elution with two mobile phases: 0.1% v/v formic acid (aqueous solution) and 0.1% v/v formic acid (acetonitrile solution). The linearity of the method was demonstrated within a concentration range of 0.5–20 ng/mL, with correlation coefficients between 0.998–0.999 for goserelin, buserelin, triptorelin, and octreotide, respectively. The relative standard deviation (RSD, %) values for method accuracy and precision did not exceed 20% at the lower level of quantitation (LLOQ) or 15% at other concentration levels.

Published in Molecules

ISSN: 1420-3049 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Chemistry: Organic chemistry
Website: http://www.mdpi.com/journal/molecules

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