Bioremediation of Neonicotinoid Pesticide, Imidacloprid, Mediated by <i>Bacillus cereus</i>

Farah Naz Talpur; Ahsanullah Unar; Sana Kanwal Bhatti; Laila Alsawalha; Dalia Fouad; Humaira Bashir; Hassan Imran Afridi; Farid Shokry Ataya; Ohoud A. Jefri; Muhammad Sohail Bashir

doi:10.3390/bioengineering10080951

Bioengineering (Aug 2023)

Bioremediation of Neonicotinoid Pesticide, Imidacloprid, Mediated by <i>Bacillus cereus</i>

Farah Naz Talpur,
Ahsanullah Unar,
Sana Kanwal Bhatti,
Laila Alsawalha,
Dalia Fouad,
Humaira Bashir,
Hassan Imran Afridi,
Farid Shokry Ataya,
Ohoud A. Jefri,
Muhammad Sohail Bashir

Affiliations

Farah Naz Talpur: National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro 76080, Pakistan
Ahsanullah Unar: School of Life Sciences, University of Sciences and Technology of China, Hefei 230052, China
Sana Kanwal Bhatti: National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro 76080, Pakistan
Laila Alsawalha: Faculty of Science, Zarqa University, Zarqa 13110, Jordan
Dalia Fouad: Department of Zoology, College of Science, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi Arabia
Humaira Bashir: Department of Botany, University of the Punjab, Quaid-e-Azam Campus, 54590 Lahore, Pakistan
Hassan Imran Afridi: National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro 76080, Pakistan
Farid Shokry Ataya: Department of Biochemistry, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia
Ohoud A. Jefri: Department of Biological Science, Faculty of Science, King Abdul-Aziz University, Jeddah 2158, Saudi Arabia
Muhammad Sohail Bashir: Institutes of Physical Science and Information Technology, Key Laboratory of Structure and Functional Regulation of Hybrid Materials of Ministry of Education, Anhui University, Hefei 230601, China

DOI: https://doi.org/10.3390/bioengineering10080951
Journal volume & issue: Vol. 10, no. 8
p. 951

Abstract

Read online

Imidacloprid, a toxic pesticide of the chloronicotinyl category, is employed extensively in agricultural fields, and its exposure causes serious health issues. Biodegradation is considered to be a green and economical approach to remediate pesticides. Herein, imidacloprid degradation efficiency of Bacillus sp. is highlighted, among which Bacillus cereus exhibited the greatest degradation; optimization of experimental variables (pH, imidacloprid and agitation time) via Box–Behnken factorial design and analysis of variance (ANOVA) revealed 92% biodegradation at the initial substrate concentration of 0.03 mM, aerobically in 11 days under favorable pH 7. The subsequent metabolites, identified through liquid chromatography–mass spectrometry, were 5-hydroxy imidacloprid, imidacloprid-guanidine and 6-chloronicotinic acid.

Published in Bioengineering

ISSN: 2306-5354 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Technology; Science: Biology (General)
Website: https://www.mdpi.com/journal/bioengineering

About the journal

Abstract

Keywords