WaveMAP for identifying putative cell types from in vivo electrophysiology

Abstract

Summary: Action potential spike widths are used to classify cell types as either excitatory or inhibitory; however, this approach obscures other differences in waveform shape useful for identifying more fine-grained cell types. Here, we present a protocol for using WaveMAP to generate nuanced average waveform clusters more closely linked to underlying cell types. We describe steps for installing WaveMAP, preprocessing data, and clustering waveform into putative cell types. We also detail cluster evaluation for functional differences and interpretation of WaveMAP output.For complete details on the use and execution of this protocol, please refer to Lee et al. (2021).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Keywords

• Cognitive Neuroscience
• Behavior