Nature Communications (Jan 2019)
Early preclinical detection of prions in the skin of prion-infected animals
- Zerui Wang,
- Matteo Manca,
- Aaron Foutz,
- Manuel V. Camacho,
- Gregory J. Raymond,
- Brent Race,
- Christina D. Orru,
- Jue Yuan,
- Pingping Shen,
- Baiya Li,
- Yue Lang,
- Johnny Dang,
- Alise Adornato,
- Katie Williams,
- Nicholas R. Maurer,
- Pierluigi Gambetti,
- Bin Xu,
- Witold Surewicz,
- Robert B. Petersen,
- Xiaoping Dong,
- Brian S. Appleby,
- Byron Caughey,
- Li Cui,
- Qingzhong Kong,
- Wen-Quan Zou
Affiliations
- Zerui Wang
- Department of Pathology, Case Western Reserve University School of Medicine
- Matteo Manca
- Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH)
- Aaron Foutz
- National Prion Disease Pathology Surveillance Center, Case Western Reserve University School of Medicine
- Manuel V. Camacho
- Department of Pathology, Case Western Reserve University School of Medicine
- Gregory J. Raymond
- Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH)
- Brent Race
- Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH)
- Christina D. Orru
- Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH)
- Jue Yuan
- Department of Pathology, Case Western Reserve University School of Medicine
- Pingping Shen
- Department of Pathology, Case Western Reserve University School of Medicine
- Baiya Li
- Department of Pathology, Case Western Reserve University School of Medicine
- Yue Lang
- Department of Pathology, Case Western Reserve University School of Medicine
- Johnny Dang
- Department of Pathology, Case Western Reserve University School of Medicine
- Alise Adornato
- Department of Pathology, Case Western Reserve University School of Medicine
- Katie Williams
- Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH)
- Nicholas R. Maurer
- Department of Pathology, Case Western Reserve University School of Medicine
- Pierluigi Gambetti
- Department of Pathology, Case Western Reserve University School of Medicine
- Bin Xu
- Department of Biochemistry, Virginia Polytechnic Institute and State University
- Witold Surewicz
- Department of Physiology and Biophysics, Case Western Reserve University School of Medicine
- Robert B. Petersen
- Department of Pathology, Case Western Reserve University School of Medicine
- Xiaoping Dong
- State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention
- Brian S. Appleby
- Department of Pathology, Case Western Reserve University School of Medicine
- Byron Caughey
- Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH)
- Li Cui
- Department of Neurology, The First Hospital of Jilin University
- Qingzhong Kong
- Department of Pathology, Case Western Reserve University School of Medicine
- Wen-Quan Zou
- Department of Pathology, Case Western Reserve University School of Medicine
- DOI
- https://doi.org/10.1038/s41467-018-08130-9
- Journal volume & issue
-
Vol. 10,
no. 1
pp. 1 – 11
Abstract
There are currently no validated methods for the diagnosis of prion disease at the preclinical stage. Here the authors show that serial protein misfolding cyclic amplification and real-time quaking-induced conversion can be used to detect prions in the skin of prion-inoculated hamsters and humanized transgenic mice at early preclinical stages.
