Jichu yixue yu linchuang (Jun 2023)

Enolase1 regulates the self-renewal ability of human embryonic stem cells by influencing mRNA localization

  • HE Jiayin, YANG Jiabin, CHEN Zhongyang, MA Yanni, YU Jia

DOI
https://doi.org/10.16352/j.issn.1001-6325.2023.06.0867
Journal volume & issue
Vol. 43, no. 6
pp. 867 – 874

Abstract

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Objective To explore the effect of enolase1 (ENO1) expression on maintaining the self-renewal activity of human embryonic stem cells (hESCs) and reveals its mechanism. Methods RNAs and proteins bound to ENO1 were predicted and analyzed through Gene Oncology (GO) enrichment. Endogenous expression of ENO1 was inhibited by shRNA in hESCs. The colony forming of hESCs was examined by clonogenesis and alkaline phosphatase (AP) staining experiments. The pluripotency and differentiation marker gene expression in hESCs were detected by qPCR. RNA fluorescence in situ hybridization was used to detect the localization changes of poly (A) + RNAs. Results Inhibition of ENO1 endogenous expression significantly inhibited colony forming of hESCs (P＜0.05)and the expression of differentiation marker genes was increased (P＜0.001). Localization of poly(A)+ RNA changed from cytoplasm to nucleus (P＜0.01) with ENO1 inhibition. Conclusions ENO1 regulates the activity of hESCsself-renew by regulating mRNA localization.

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