Engineering inducible biomolecular assemblies for genome imaging and manipulation in living cells

Qin Peng; Ziliang Huang; Kun Sun; Yahan Liu; Chi Woo Yoon; Reed E. S. Harrison; Danielle L. Schmitt; Linshan Zhu; Yiqian Wu; Ipek Tasan; Huimin Zhao; Jin Zhang; Sheng Zhong; Shu Chien; Yingxiao Wang

doi:10.1038/s41467-022-35504-x

Nature Communications (Dec 2022)

Engineering inducible biomolecular assemblies for genome imaging and manipulation in living cells

Qin Peng,
Ziliang Huang,
Kun Sun,
Yahan Liu,
Chi Woo Yoon,
Reed E. S. Harrison,
Danielle L. Schmitt,
Linshan Zhu,
Yiqian Wu,
Ipek Tasan,
Huimin Zhao,
Jin Zhang,
Sheng Zhong,
Shu Chien,
Yingxiao Wang

Affiliations

Qin Peng: Department of Bioengineering, Institute of Engineering in Medicine, University of California
Ziliang Huang: Department of Bioengineering, Institute of Engineering in Medicine, University of California
Kun Sun: Institute of Cancer Research, Shenzhen Bay Laboratory
Yahan Liu: Department of Bioengineering, Institute of Engineering in Medicine, University of California
Chi Woo Yoon: Department of Bioengineering, Institute of Engineering in Medicine, University of California
Reed E. S. Harrison: Department of Bioengineering, Institute of Engineering in Medicine, University of California
Danielle L. Schmitt: Department of Pharmacology, University of California
Linshan Zhu: Department of Bioengineering, Institute of Engineering in Medicine, University of California
Yiqian Wu: Department of Bioengineering, Institute of Engineering in Medicine, University of California
Ipek Tasan: Department of Biochemistry, University of Illinois at Urbana-Champaign
Huimin Zhao: Department of Biochemistry, University of Illinois at Urbana-Champaign
Jin Zhang: Department of Pharmacology, University of California
Sheng Zhong: Department of Bioengineering, Institute of Engineering in Medicine, University of California
Shu Chien: Department of Bioengineering, Institute of Engineering in Medicine, University of California
Yingxiao Wang: Department of Bioengineering, Institute of Engineering in Medicine, University of California

DOI: https://doi.org/10.1038/s41467-022-35504-x
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 15

Abstract

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Imaging non-repetitive loci in living cells remains challenging. Here, the authors engineered an inducible system whereby biomolecular assemblies can be guided to specific genomic loci by a nuclease-defective Cas9, allowing the simultaneous imaging and manipulation of the loci.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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