Frequency of BCR-ABL 1 Fusion Transcripts Variants in Chronic Myeloid Leukemia Algerian patients

Abstract

Aim - In chronic myeloid leukemia (CML), the research of BCR-ABL1 fusion transcript became essential to confirm the molecular diagnosis. In this study, we describe the different types of BCR-ABL1 fusion transcripts found in CML patients in West Algerian patients. Methods - A total of 167 CML suspected patients were included in our study. A qualitative research on fusion transcripts was carried out by reverse transcription polymerase chain reaction (RT-PCR) technique in the biochemistry laboratory of Oran’s university hospital establishment. Results. The two types of major BCR-ABL1 fusion transcript Mb3a2 and Mb2a2 were present in 59,8 % and 36,4% of cases respectively. Two patients (1,8%) had an atypical and rare transcript type e19a2 and two others (1,8%) co-expressed b3a2 and b2a2 transcript types. Conclusion - Our study confirmed the data in the literature with a higher incidence of the major transcript.

Keywords

• Chronic Myeloid Leukemia
• multiplex RT-PCR
• BCR-ABL1Transcript
• rare fusion transcripts
• Co-expression b3a2/ b2a2