Biological characterization of introgressive barley lines obtained on the basis of the interspecific hybrid Hordeum vulgare L. × H. bulbosum L. (HvHbHb)

Abstract

Background. Involving alien genetic material Hordeum bulbosum in the genome for the expansion of the genetic diversity of barley is a cultural important task because this species is characterized by a number of valuable traits. One way of using the genetic potential of bulbous barley is interspecies hybridization and the production of fertile introgressive lines of H. vulgare on the base of interspecies hybrids. The purpose of our investigation was to study introgressive lines of cultural barley obtained on the basis of the triploid hybrid H.vulgare cv 'Igri' (2x) × H. bulbosum (4x) in compare to the original Igri variety in the field conditions.Materials and methods. In the field conditions the barley lines with intro-gressions of the genetic material of bulbous barley in the different arms of chromosomes H. vulgare (1HL, 2HL, 3HL, 5HL, 7HL, 2HS, 1HL + 5HL) were studied. Methods. In situ hybridization (FISH, GISH) was used to identify the introgressions and to analyze their saving in line karyotypes after field reproduction. For cultivated in field conditions plants the estimation of winter survival and graininess were estimated. Structural analysis of plants was carried out on tillers of the main spike after maturation. The quality of the grain was determined by the non-destructive method of monitoring - near infrared spectroscopy, using the Infralum FT-10 BIC analyzer.Results. Closed flowering and self-pollination were observed for plants of the studied lines same as for parent variety. The introgressions were saved in the offspring when the plants cultivated in a field conditions without isolation of spike. The analysis showed that small fragments of chromosomes of H. bulbosum in chromosomes 1HL, 2HL, 3HL, 5HL,7HL, 1HL + 5HL of the studied lines do not significantly affect on characteristics (of fertility, productivity and grain quality) of the variety. Differences from the initial variety were observed at line 14.10 with the introgression of genetic material in the chromosome 2HS. It is characterized by lower fertility and productivity, and protein content in the grain is higher than that of the original variety.Conclusions. The studied lines are highfertile forms of barley. The introgression of the genetic material of H. bulbosum into the terminal region of the short arm of the chromosome 2H causes changes in some characteristics of the Igri variety.

Keywords

• barley
• hordeum vulgare
• hordeum bulbosum
• interspesific hybridization
• alien introgression
• introgressive line