GENETIC POLYMORHISM OF CASPASE 8 AND 9 IN IRAQ

Abstract

This study examined the potential association between polymorphisms of Caspase 9 −293del (rs4645982) and Caspase8−652 6N ins / del (rs3834129) and the danger of acute and chronic myeloid leukaemia in Iraqi population. This case-control study was conducted as 128 (62 acute, and 66 chronic) myeloid leukaemia patient and 65 healthy individuals not infected with cancer. A conventional PCR method was used for detection in this study. The results showed that the Caspase9 −293del (rs4645982) in homozygous and heterozygous deletion genotypes were associated significantly with CML risk ( p= 0.001 and 0.005 ,respectively ) and in homozygous insertion and deletion genotypes of Caspase 8 -652 6N were also associated significantly with CML risk (p= 0.004 and p= 0.00, respectively), while there were non significant association between Caspase9 −293del (rs4645982) and Caspase8 −652 6N (rs3834129) with AML risk in all genotypes of them p > 0.05. These findings suggest that polymorphisms in the Caspase-9 and Caspase-8 genes might be useful markers for determining genetic susceptibility to CML and AML. The results of this study suggest that Caspase9-293 is associated with increased risk of CML in case of homozygous of deletion, and insertion in case of AML, while in Caspase8-652 6N insertion polymorphism are associated with increased risk of both CML and AML in a sample of Iraqi populations.

Keywords

• Acute myeloid leukemia, Chronic myeloid leukemia, Apoptosis, Odd ratio