Next-generation unnatural monosaccharides reveal that ESRRB O-GlcNAcylation regulates pluripotency of mouse embryonic stem cells

Yi Hao; Xinqi Fan; Yujie Shi; Che Zhang; De-en Sun; Ke Qin; Wei Qin; Wen Zhou; Xing Chen

doi:10.1038/s41467-019-11942-y

Nature Communications (Sep 2019)

Next-generation unnatural monosaccharides reveal that ESRRB O-GlcNAcylation regulates pluripotency of mouse embryonic stem cells

Yi Hao,
Xinqi Fan,
Yujie Shi,
Che Zhang,
De-en Sun,
Ke Qin,
Wei Qin,
Wen Zhou,
Xing Chen

Affiliations

Yi Hao: College of Chemistry and Molecular Engineering, Peking University
Xinqi Fan: College of Chemistry and Molecular Engineering, Peking University
Yujie Shi: College of Chemistry and Molecular Engineering, Peking University
Che Zhang: College of Chemistry and Molecular Engineering, Peking University
De-en Sun: College of Chemistry and Molecular Engineering, Peking University
Ke Qin: College of Chemistry and Molecular Engineering, Peking University
Wei Qin: College of Chemistry and Molecular Engineering, Peking University
Wen Zhou: College of Chemistry and Molecular Engineering, Peking University
Xing Chen: College of Chemistry and Molecular Engineering, Peking University

DOI: https://doi.org/10.1038/s41467-019-11942-y
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 13

Abstract

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Per-O-acetylated unnatural monosaccharides are popular tools for glycan labeling in live cells but can undergo unwanted side reactions with cysteines. Here, the authors develop unnatural sugars in a partially esterified form that are inert towards cysteines, and use them to probe O-GlcNAcylation in mESCs.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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