In vitro hepatic biotransformation of the algal toxin pectenotoxin-2

Morten Sandvik; Christopher O. Miles; Alistair L. Wilkins; Christiane Fæste

Toxicon: X (Jun 2020)

In vitro hepatic biotransformation of the algal toxin pectenotoxin-2

Morten Sandvik,
Christopher O. Miles,
Alistair L. Wilkins,
Christiane Fæste

Affiliations

Morten Sandvik: Norwegian Veterinary Institute, P. O. Box 750 Sentrum, NO-0106, Oslo, Norway; Corresponding author.
Christopher O. Miles: Norwegian Veterinary Institute, P. O. Box 750 Sentrum, NO-0106, Oslo, Norway; Biotoxin Metrology, Measurement Science and Standards, National Research Council, 1411 Oxford Street, Halifax, NS, B3H 3Z1, Canada
Alistair L. Wilkins: Norwegian Veterinary Institute, P. O. Box 750 Sentrum, NO-0106, Oslo, Norway; Waikato University, Private Bag 3105, Hamilton, 3240, New Zealand
Christiane Fæste: Norwegian Veterinary Institute, P. O. Box 750 Sentrum, NO-0106, Oslo, Norway

Journal volume & issue: Vol. 6

Abstract

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We have investigated the in vitro metabolism of pectenotoxin-2 (PTX-2) using primary hepatocytes from Wistar rats in suspension. Purified PTX-2 was rapidly metabolized. Two major and several minor oxidized PTX-2 metabolites were formed, none of which had retention times corresponding to PTX-1, -11, or −13. Hydrolysis products, such as PTX-2 seco acid, were not observed. Preliminary multi-stage LC-MS analyses indicated that the major hepatic PTX-2 metabolites resulted from the insertion of an oxygen atom at the positions C-19 to C-24, or at C-44. The rapid oxidative metabolism may explain the low oral toxicity of PTXs observed in vivo studies.

Published in Toxicon: X

ISSN: 2590-1710 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Medicine: Public aspects of medicine: Toxicology. Poisons
Website: https://www.journals.elsevier.com/toxicon-x

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